Abcam, ab171179, Mouse IL-12 p40 ELISA Kit

Size: 1 x 96Tests
Mouse IL-12 p40 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-12 p40 in Mouse in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 10 pg/mL,
Range:15.6 - 1000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's mouse IL-12 p40
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse IL-12 p40 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
A IL-12 p40 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for IL-12 p40 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow coloration after adding acidic stop solution. The density of yellow is directly proportional to the mouse IL-12 p40 amount of sample captured in plate.
The standard is affinity purified.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C