Abcam, ab172187, Recombinant Human PRP19 protein - BSA and Azide free

Size: 20µg / 50µg
Recombinant Human PRP19 protein - BSA and Azide free is a Human Full Length protein, in the 2 to 504 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB.
Key facts
Purity:>90% Densitometry,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9UMS4,
Animal free:No,
Carrier free:Yes,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PRP19 protein also known as Pre-mRNA Processing factor 19 weighs approximately 55 kDa. This protein plays a role in the splicing of pre-mRNA by functioning as part of the spliceosome complex. PRP19 is expressed in many tissues and cells which reflects its important role in RNA processing. It is not confined to a specific type of tissue indicating its broad relevance in cellular processes. Mechanically PRP19 acts by enhancing the efficiency of splicing ensuring that mRNA molecules are properly matured for translation.
Biological function summary
PRP19 engages in multiple processes beyond its initial splicing role. It forms part of the NTC complex which is critical for the activation of the spliceosome. This complex includes other key proteins essential for RNA splicing transition phases. Additionally PRP19 contributes to DNA repair mechanisms playing a part in maintaining genomic integrity under stress conditions. Its role in these processes demonstrates its versatile functionality in cell homeostasis and response to damage.
Pathways
Experts recognize PRP19's involvement in the mRNA processing and DNA damage response pathways. These pathways are key to understanding how cells manage genetic information and react to physiological challenges. PRP19 interacts with other proteins such as CDC5L and PLRG1 within these pathways illustrating its interconnected role in cellular regulation. These associations underline how PRP19 integrates into critical networks that balance gene expression and genome stability.
Researchers have linked PRP19 to cancer and neurodegenerative disorders. In cancer abnormal PRP19 function may contribute to unchecked cell growth by impacting mRNA splicing and DNA repair. In neurodegenerative diseases its dysfunction might influence neuron survival through defective RNA processing. The relationship of PRP19 with proteins like BRCA1 in the context of cancer further highlights its significance in disease mechanisms. As such PRP19 serves as a potential target for therapeutic exploration.