Abcam, ab172382, Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free

Size: 100µg / 1mg
Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting LRRK2 in Western Blot . Suitable for Human, Mouse . - BSA, sodium azide, and glycerol-free for easy conjugation - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:UDD2 10(12),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The antibody does not give a positive signal in U-87 MG, SH-SY-5Y and human fetal brain. Please contact our Scientific Support team if you have any question.

Product details:
What is this antibody validated in?
Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse samples.
What is the molecular weight of LRRK2?
Anti-LRRK2 (phospho S935) [UDD2 10(12)] - BSA and Azide free (ab172382) specifically detects a band for LRRK2 (UniProt: Q5S007) at a molecular weight of 286kDa.
Trusted by the scientific community
Anti-LRRK2 (phospho S935) [UDD2 10(12)] - BSA and Azide free (ab172382) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals.
Other related products
We have a range of other formats of antibody clone [UDD2 10(12)] also available for your convenience:
ab133450
, Carrier free - ab172382, Carrier free -
ab324096
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.
Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein LRRK2 also known as leucine-rich repeat kinase 2 or dardarin is an enzyme with a molecular weight of approximately 286 kDa. It functions as a kinase meaning it adds phosphate groups to other proteins which affects their activity. LRRK2 is expressed in various tissues but it is highly abundant in the brain especially in regions such as the striatum and cortex. It has a significant role in cellular signaling processes due to its phosphorylation activity.
Biological function summary
LRRK2 interacts with cellular mechanisms by regulating cytoskeletal dynamics autophagy and vesicle trafficking. It is a part of a larger complex that includes other proteins involved in these processes. The kinase activity of LRRK2 plays an essential part in maintaining neuronal health and function. It influences the process of autophagy which is a way cells clean themselves by removing damaged components and recycling them.
Pathways
The action of LRRK2 is central to the mitogen-activated protein kinase (MAPK) and the mammalian target of rapamycin (mTOR) pathways. In these pathways LRRK2 interacts with other proteins such as mTOR and RPS6KB1. It modulates cellular processes like growth proliferation and response to stressors. Its kinase activity affects the phosphorylation state of targets within the pathways hence influencing biological outcomes like survival and apoptosis.
LRRK2 mutations have a significant connection to Parkinson's disease and Crohn's disease. In Parkinson's disease mutated LRRK2 leads to abnormal protein aggregation linking to proteins such as alpha-synuclein. For Crohn's disease LRRK2 influences the immune response and intestinal inflammation. These connections highlight LRRK2's role in the pathogenesis and contribute to understanding these complex disorders.