Abcam, ab172962, Anti-PICALM antibody [EPR12177]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal PICALM antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12177,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PICALM also known as Phosphatidylinositol binding clathrin assembly protein is a protein weighing approximately 70 kDa. It plays an important role in clathrin-mediated endocytosis where it helps assembly of the clathrin coat on the cytoplasmic face of the cell membrane. Expression of the PICALM gene is found in various tissues with significant levels in the brain lungs and spleen. The gene encoding PICALM is located on chromosome 11.
Biological function summary
PICALM is essential in the trafficking of molecules within cells particularly in the endocytosis and endosomal sorting pathways. This protein is part of large protein complexes assembled during the formation of vesicles. Through these complexes PICALM influences the transport and recycling of membrane receptors and other molecules ensuring proper cellular functions. The clathrin coat assembly and disassembly rely heavily on its function for effective cellular trafficking processes.
Pathways
PICALM integrates into the clathrin-mediated endocytosis and vesicle-mediated transport pathways. It works alongside proteins such as adaptin and dynamin to facilitate the internalization of proteins and later processing within cellular compartments. In the context of cellular signaling and homeostasis these pathways involving PICALM contribute significantly to receptor recycling and downregulation maintaining proper cellular responses to external stimuli.
PICALM has been linked to Alzheimer's disease and acute myeloid leukemia (AML). In Alzheimer's disease altered PICALM function or expression can affect amyloid precursor protein processing implicating proteins such as presenilins and tau as well. For acute myeloid leukemia the PICALM-MLLT10 fusion protein resulting from chromosomal translocations can contribute to leukemogenesis by disrupting normal gene regulation. These associations highlight the diverse roles and importance of PICALM in both normal physiology and pathology.