Abcam, ab174284, Anti-Smac/Diablo antibody [EPR10934]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Smac/Diablo antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10934,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Smac/Diablo is a mitochondrial protein that promotes apoptosis by interfering with inhibitor of apoptosis proteins (IAPs). The protein also known as Second Mitochondria-derived Activator of Caspases weighs approximately 27 kDa. Researchers detect Smac/Diablo in various tissues with higher expression levels in organs like the heart and brain. This protein's expression also varies depending on the cell's state and external stimuli.
Biological function summary
Smac/Diablo plays a significant role in programmed cell death by binding to IAPs and negating their inhibition of caspases the key executors of apoptosis. Smac/Diablo releases from mitochondria into the cytosol when apoptotic signals activate. It does not form complexes but interacts with IAPs facilitating the activation of caspases and enhancing the apoptotic response. Its interaction with IAPs highlights its important function in apoptosis regulation.
Pathways
Smac/Diablo integrates into the mitochondrial apoptosis pathway contributing to the intrinsic pathway of apoptosis. It closely interacts with proteins such as caspases and IAPs. This integration is important for apoptosis regulation linking mitochondrial outer membrane permeabilization with the activation of caspases. Additionally the protein engages in the cytochrome c pathway promoting apoptosis by restricting IAPs and aiding in the release of cytochrome c important for apoptosis progression.
Researchers associate Smac/Diablo with cancer and neurodegenerative diseases like Alzheimer's disease. In cancer elevated Smac/Diablo levels can result in increased apoptotic death of cancerous cells potentially serving as a target for therapy. It also interacts with proteins like XIAP in these disease contexts. In neurodegenerative disorders dysregulation of apoptosis pathways involving Smac/Diablo might contribute to excessive neuronal cell death. Understanding Smac/Diablo's role in these diseases provides insights into possible therapeutic interventions.