Abcam, ab174446, Human RANTES ELISA Kit (CCL5)

Size: 1 x 96Tests
Human RANTES ELISA Kit (CCL5) is a single-wash 90-min Simplestep used to quantify Human RANTES (CCL5) with a sensitivity of 0.091 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 15 citations
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.091 pg/mL,
Range:0.94 - 60 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
Human RANTES ELISA Kit (CCL5) ab174446 is a rapid single-wash 90-min Sandwich ELISA to measure Human RANTES (CCL5)in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, plasma, serum. This SimpleStep sensitivity is 0.091 pg/mL.
How the assay works
Human RANTES (CCL5)SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human RANTES ELISA Kit (CCL5) ab174446 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-RANTES antibody [EPR20534-97] - BSA and Azide free (Capture)
ab244884
- Anti-RANTES antibody [EPR20534-199] - BSA and Azide free (Detector)
ab244884
RANTES (Regulated on Activation, Normal T cell Expressed and Secreted) is a chemotactic cytokine for T cells, eosinophils, and basophils. RANTES also acts as a recruitment signal for leukocytes into inflammatory sites with the help of cyotokines (IL-2 and IFN-γ) released by CD8+ T cells. RANTES also activates and induces proliferation of natural-killer cells to form CHAK (CC-Chemokine-activated killer) cells. RANTES has also been identified to inhibit infection of HIV.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C