Abcam, ab174447, Human Clusterin ELISA Kit

Size: 1 x 96Tests
Human Clusterin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Clusterin with a sensitivity of 22.5 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Urine, Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma,
Reacts with:Human, Rhesus monkey,
Assay type:Sandwich (quantitative),
Sensitivity:= 22.5 pg/mL,
Range:234.4 - 15000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Clusterin ELISA Kit ab174447 is a rapid single-wash 90-min ELISA kit to measure Human Clusterin in saliva, urine, cell culture extracts, cell culture supernatant, milk, serum, and plasma. This SimpleStep ELISA® sensitivity is 22.5 pg/mL.
How the assay works
Human Clusterin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human Clusterin protein in serum, plasma, urine, saliva, milk, cell culture supernatant, and cell extract samples only. Tissue extract samples have not been tested with this kit. This kit was found to recognize the alpha chain of the Clusterin protein.
Human Clusterin ELISA Kit ab174447 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human Clusterin ELISA Kit ab174447
Human Clusterin ELISA Kit ab174447 has been used to study the role of clusterin in regulating fibroblasts in COPD
, metal homeostasis in age-related macular degeneration
, and the potential of clusterin as a diagnostic and prognostic marker for osteoporosis
References:
Rui Zheng et al. 2022, PMID: 35191419,
Hйctor Gonzбlez-Iglesias et al. 2022, PMID: 35426907,
Umberto Tarantino et al. 2022, PMID: 35456459
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human/Monkey Clusterin Antibody Pair - BSA and Azide free
ab243972
- Anti-Clusterin antibody [EPR20729-240] - BSA and Azide free (Capture)
ab244626
- Anti-Clusterin antibody [EPR20729-41] - BSA and Azide free (Detector)
ab244885
Related and recommended products
Human Clusterin ELISA Kit ab174447 is commonly used to study metabolism and neurodegenerative diseases.
Other kits to measure lipid metabolism and neurodegeneration include:
- Human Leptin ELISA Kit
ab179884
- Human Apo E ELISA Kit
ab233623
- Human TREM2 ELISA Kit
ab224881
Clusterin (CLU) is composed of an antiparallel, disulfide-linked heterodimer of an alpha chain and a beta chain that self-associate and can form higher oligomers. It is ubiquitously expressed in various tissues. Clusterin functions as an extracellular chaperone that prevents aggregation of nonnative proteins. It does not require ATP and does not refold proteins by itself. Clusterin does so by maintaining partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Clusterin prevents stress-induced aggregation of blood plasma proteins by inhibiting the formation of amyloid fibrils through APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). It can bind to cell surface receptors and trigger internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Clusterin also known as Apolipoprotein J (ApoJ) is a glycoprotein with a mass of approximately 75-80 kDa. Scientists find clusterin expressed widely in the human body including the brain kidney and reproductive organs. This protein exists in two isoforms a secreted form and a nuclear form each with different locations and functions within the cells.
Biological function summary
Clusterin plays multiple roles particularly in lipid transport apoptosis and cell-cell interactions. It does not form part of a larger protein complex but it acts as a molecular chaperone that helps in folding and clearance of damaged proteins. This capability allows clusterin to protect cells against stress conditions such as oxidative stress by preventing abnormal protein aggregation.
Pathways
Clusterin is involved in pathways related to cell survival and lipid metabolism. It plays a role in the insulin signaling pathway and interacts with proteins like Heat Shock Protein 70 (HSP70) to assist in protein folding and in the activation of apoptotic pathways when needed. Clusterin's function in these processes helps in maintaining cellular homeostasis.
Scientists associate clusterin with Alzheimer’s disease and cancer progression. Elevated levels of clusterin have been observed in Alzheimer's patients suggesting a role in amyloid-beta plaque formation. In cancer clusterin expression influences tumor progression and resistance to therapies. Its interaction with other proteins such as Bax modulates the apoptotic pathways impacting cancer cell survival and chemotherapy resistance.