Abcam, ab174633, Anti-Synaptotagmin VII antibody [N275/14]

Size: 100µg
Mouse Monoclonal Synaptotagmin VII antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Synaptotagmin-7 aa 150-250.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:N275/14,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Rat, Human,
Applications:IHC-P, IHC-Fr, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Mouse Synaptotagmin-7 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.Q9R0N7,
Epitope:Cytoplasmic C2A domain,
Specificity:Does not cross-react with Synaptotagmin VI (or others).

Product details:
Abcam is committed to ensuring that our customers have access to all of the information we have on our products and that the product information is accurate. The clone name for this product was previously known as S27514. However, it has come to our attention that the original and primary clone name is N275/14 so we have therefore changed the product name accordingly.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Synaptotagmin VII also known as Syt7 is a calcium-sensitive protein that plays an important role in the process of vesicle trafficking and membrane fusion. It has a molecular weight of approximately 48 kDa. This protein belongs to the synaptotagmin family with several isoforms expressed in different tissues. Synaptotagmin VII is mainly found in the cerebrum and other parts of the central nervous system where it regulates neurotransmitter release. Its calcium-binding domains are essential for its function in synaptic vesicle exocytosis and endocytosis.
Biological function summary
The function of Synaptotagmin VII involves the facilitation of calcium-dependent exocytosis. It acts as a calcium sensor that triggers the rapid release of neurotransmitters in response to synaptic activity. Synaptotagmin VII forms part of a larger protein complex that includes SNARE proteins which are directly involved in the docking and fusion of synaptic vesicles. Synaptotagmin VII's action ensures efficient communication between neurons by ensuring that neurotransmitter release occurs at the right moment when triggered by a rise in intracellular calcium levels.
Pathways
Synaptotagmin VII is important for pathways that involve synaptic vesicle cycling which includes the calcium-triggered synaptic vesicle exocytosis pathway. It interacts with other proteins such as syntaxin and SNAP-25 as part of the SNARE complex which mediates vesicle fusion. Synaptotagmin VII's function in these pathways ensures that neuronal signaling is rapid and tightly regulated a vital component for maintaining synaptic plasticity and normal brain function.
Alterations in Synaptotagmin VII expression or function can be linked to neurological conditions such as epilepsy and certain forms of cognitive dysfunction. Dysregulation of Synaptotagmin VII may lead to impaired neurotransmission contributing to the pathophysiology of these disorders. Moreover its connection with proteins in the SNARE complex hints at its potential involvement in diseases where these pathways are disrupted. For instance an imbalance in synaptotagmin and SNARE protein interactions can lead to defective neurotransmitter release potentially exacerbating synaptic abnormalities observed in some seizure disorders.