Abcam, ab176112, Human Frataxin ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Human Frataxin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Frataxin with a sensitivity of 2.1 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 384-well plate for higher throughput - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Tissue Extracts,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.1 pg/mL,
Range:9.88 - 800 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
Human Frataxin ELISA Kit ab176112 is a rapid single-wash 90-min ELISA kit to measure Human Frataxin in cell culture extracts and tissue extracts. This SimpleStep ELISA® sensitivity is 2.1 pg/mL.
How the assay works
Human Frataxin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human Frataxin protein in cell and tissue extract samples only. Serum, plasma, saliva, and urine samples have not been tested with this kit.
Human Frataxin ELISA Kit ab176112 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human Frataxin ELISA Kit ab176112
Human Frataxin ELISA Kit ab176112 has been used to study effects and mechanism of Friedreich ataxia including cardiac mitochondrial stress
, treatment with trans-activator of transcription peptides
, and treatment with cytokine therapy
References:
Giovanni Manfredi et al. 2023, PMID: 37691621,
Joaquim Ros et al. 2018, PMID: 28980774,
Alastair Wilkins et al. 2017, PMID: 28009062
Save your precious samples
Our 384-well Human Frataxin ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Frataxin Antibody Pair - BSA and Azide free
ab256593
- Anti-Frataxin antibody [30-17A11AC7] - BSA and Azide free (Capture)
ab259433
- Anti-Frataxin antibody [30-18A5DB1] - BSA and Azide free (Detector)
ab259434
Related and recommended products
Human Frataxin ELISA Kit ab176112 is commonly used to study Friedreich's ataxia, a neurodegenerative disease caused by frataxin mutations leading to oxidative stress.
Other products to measure frataxin include:
- Mouse Frataxin ELISA Kit
ab199078
- Anti-Frataxin antibody [17A11]
ab113691
- Recombinant Human Frataxin protein
ab95502
Frataxin is a 17 kDa nuclear-encoded mitochondrial protein. In humans the gene is localized on chromosome 9 and is highly conserved during evolution. The gene is expressed in every cell, although in varying levels in different tissues and during development. The specific function of frataxin is still unknown, but it has been shown to play a role in iron metabolism. Studies have demonstrated that the deletion of the frataxin gene in yeast results in iron accumulation in mitochondria and loss of respiration. Recombinant human frataxin has been shown to bind iron in vitro, and increased mitochondrial iron levels have been observed in patients with Friedreich's Ataxia (FRDA). FRDA is an autosomal recessive, progressive degenerative disease characterized by neurodegeneration and cardiomyopathy it is the most common inherited ataxia.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Frataxin often known by the alternate name FXN is a mitochondrial protein with a mass of approximately 21000 Dalton. It is expressed mainly in tissues with high energy demands like the heart liver and pancreas. Frataxin plays an important role in iron-sulfur cluster assembly which is essential for various cellular processes. The protein is a part of mitochondria where it regulates iron homeostasis and prevents oxidative damage by minimizing iron-induced free radical generation.
Biological function summary
Several cellular processes depend on the correct function of this protein. Frataxin assists in forming iron-sulfur clusters acting within a multiprotein complex in the mitochondria. The complex includes proteins such as ISCU which are involved in the assembly and repair of iron-sulfur clusters. These clusters are necessary for supporting mitochondrial electron transport and other fundamental metabolic pathways that require iron-sulfur dependencies.
Pathways
Frataxin's involvement extensively affects the mitochondrial respiratory chain and the mitochondrial biogenesis process. It plays a role in the electron transport chain by stabilizing iron-sulfur-containing complexes. NAB is one associated protein that interacts closely within these pathways sharing a connection through iron-sulfur cluster transportation and assembly systems. Efficient function of these pathways ensures a proper energetic output of cells.
Frataxin mutations are directly linked to Friedreich's ataxia a neurodegenerative disease causing progressive damage to the nervous system. The deficiency or dysfunction in frataxin causes accumulation of iron in mitochondria leading to increased oxidative stress. Another related disorder includes heart disease which emerges due to the same oxidative stress pathway. Proteins such as Nfs1 are also involved sharing the responsibility with frataxin in scavenging excess iron protecting against related tissue damage.