Abcam, ab176279, Anti-SPCA2 antibody - N-terminal

Size: 100µg
Rabbit Polyclonal SPCA2 antibody. N-terminal. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ATP2C2.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ATP2C2. The exact immunogen used to generate this antibody is proprietary information.O75185,
Specificity:ab176279 will not cross-react with SPCA1.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SPCA2 also known as Secretory Pathway Calcium ATPase 2 is a calcium pump with a molecular mass of approximately 115 kDa. It is expressed in various tissues including the mammary gland where it is present at high levels. Mechanically SPCA2 transports calcium ions from the cytosol into the Golgi apparatus using energy from ATP hydrolysis. This helps maintain the necessary calcium concentration inside the Golgi which is important for various cellular functions.
Biological function summary
SPCA2 plays a role in calcium homeostasis necessary for proper cell function like enzyme regulation and signaling. Unlike other calcium pumps it does not form a complex with other proteins for its activity. It interacts indirectly with the calcium channel ORAI1 influencing calcium entry across the plasma membrane. This interaction means SPCA2 can partially control processes dependent on calcium signaling like those involved in secretion proliferation and differentiation.
Pathways
SPCA2 is involved in the regulation of calcium ion transport pathways contributing to overall calcium signaling pathways in cells. It indirectly affects the store-operated calcium entry (SOCE) pathway through its interaction with the ORAI1 channel. Additionally SPCA2 cooperates with proteins such as SPCA1 another calcium pump to modulate cellular calcium levels although SPCA2's unique expression patterns distinguish its specific roles.
SPCA2 has implications in breast cancer and neurodegenerative diseases. The protein demonstrates altered expression in certain types of breast cancer suggesting a role in tumor progression particularly in its estrogen receptor-positive subtypes. The interaction between SPCA2 and ORAI1 may influence this pathological development. Furthermore disruption in calcium signaling where SPCA2 plays a part is a potential factor in neurological disorders as proper calcium regulation is essential for neuronal function and survival.