Abcam, ab176725, Zinc Assay Kit (Fluorometric)

Size: 200Test
Zinc Assay Kit (Fluorometric) (ab176725) provides a robust method for detecting zinc concentration in biological samples using our proprietary Zn Detector, in which Zinc binds to the probe with enhanced fluorescence at Ex/Em= 485/525 nm.
Key facts
Detection method:Fluorescent,
Sample types:Urine, Plasma, Cell culture extracts, Tissue Extracts, Serum, Other biological fluids,
Assay type:Quantitative,
Sensitivity:>= 13 ng/mL,
Assay Platform:Microplate reader

Product details:
Zinc Assay Kit (Fluorometric) (ab176725) provides a robust method for detecting zinc concentration in biological samples using our proprietary Zn Detector, in which Zinc binds to the probe with enhanced fluorescence at Ex/Em= 485/525 nm. The Zinc probe exhibits a large increase in fluorescence in response to Zn
(greater than 200~300 folds). It has high Zn
-specificity with little responses to other metals, including Ca
, and Cu
. The assay can be used with biological samples such as serum, plasma, and urine with detection sensitivity at 0.2 μM (13 ng/mL).
Other Notes
This product was previously called Zinc Quantification Kit (Fluorometric).
Zinc is an essential trace mineral element that plays an important role in a number of biological processes. It is an essential factor required for many enzymes, protein structures, and control of genetic expression. Zinc status also affects basic processes of cell division, growth, differentiation, development, and aging. Clinical signs of zinc deficiency include acrodermatitis, low immunity, diarrhea, poor healing, stunting, hypogonadism, fetal growth failure, teratology and abortion. Simple, direct and automation-ready procedures for measuring zinc ion are highly desirable in research and drug discovery.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Zinc a mineral essential for various biological functions has a molecular mass of 65.38 Da. It is not a protein but rather a metal ion that plays a mechanical role in many proteins and enzymes. Zinc is found throughout the human body in tissues such as the brain muscle bones kidneys and liver. It is also present in high concentrations in red and white blood cells eyes and the prostate. Zinc oxide a common form sees widespread use in industries including life sciences research for applications such as the liquid zinc assay.
Biological function summary
Zinc acts as a cofactor in over 300 enzymatic reactions and is important for maintaining protein structure and function. It is part of zinc finger motifs helping in gene expression regulation by stabilizing the structure of these proteins. Zinc impacts cellular processes such as DNA repair RNA transcription and cellular signaling. It plays a role in immune system response wound healing and cell proliferation. As part of protein complexes it can bind to certain transcription factors affecting their activity and cellular response.
Pathways
Zinc influences many significant biological pathways including those related to immune response and oxidative stress. It modulates the NF-kB signaling pathway important for immune system regulation. Furthermore zinc is involved in the antioxidant defense mechanism by supporting the function of enzymes like superoxide dismutase. These pathways are linked with important proteins such as metallothioneins which regulate zinc's cellular availability and detoxify heavy metals.
Zinc deficiency correlates with conditions such as immune dysfunction and growth retardation. Its imbalance is also linked to neurodegenerative disorders like Alzheimer's disease where zinc interacts with amyloid-beta and tau proteins influencing disease progression. Zinc's interaction with proteins in these disorders suggests its levels need careful regulation to maintain health.