Abcam, ab176846, Anti-YTHDC2 antibody

Size: 100µL
Rabbit Polyclonal YTHDC2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human YTHDC2 aa 1200-1250.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human YTHDC2 aa 1200-1250. The exact immunogen used to generate this antibody is proprietary information.Q9H6S0

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab176846 was affinity purified using an epitope specific to YTHDC2 immobilized on solid support, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: 99% Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
YTHDC2 also known as YT521-B homology domain containing 2 functions as an RNA helicase with a molecular mass of approximately 160 kDa. This protein modulates RNA metabolism by promoting the efficient translation of mRNAs. It shows enriched expression in the testis as well as notable levels in immune cells and possibly in other tissues. Its involvement in the recognition and binding of N6-methyladenosine (m6A)-modified RNA substrates makes YTHDC2 a significant factor in RNA processing.
Biological function summary
YTHDC2 contributes to the regulation of mRNA stability and turnover within the cell. It may operate as part of a complex involved in post-transcriptional control. Through these actions YTHDC2 influences processes such as meiosis and the differentiation of germ cells supported by its association with m6A reader proteins. This association plays a role in the recognition of specific m6A marks critical for RNA dynamics.
Pathways
YTHDC2 integrates into the m6A-dependent RNA decay and translation machinery. One important pathway involving this protein is the m6A-mediated gene expression regulation pathway. Within this context YTHDC2 interacts closely with other m6A machinery components such as METTL3 and bridges mRNA modification with subsequent cellular outcomes. Another related pathway is spermatogenesis where the precise control by YTHDC2 is necessary for successful meiosis via interaction with various meiotic proteins.
Aberrant regulation or expression of YTHDC2 links to infertility as proper expression is necessary for normal germ cell development. This condition involves its association with other reproductive proteins such as those functioning in meiosis. Disorders in mRNA metabolism potentially leading to cancer might also implicate YTHDC2 due to its broad role in regulating mRNA stability and translation highlighting the need to understand its interactions with tumor-related pathways.