Abcam, ab177484, Anti-MAT2A + MAT1A antibody [EPR10496]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal MAT1A antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 7 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10496,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MAT2A (Methionine Adenosyltransferase 2A) and MAT1A (Methionine Adenosyltransferase 1A) are enzymes that catalyze the formation of S-adenosylmethionine (SAM) from methionine and ATP. MAT2A also known as hepatic MAT has a mass of approximately 52 kDa while MAT1A also sometimes referred to as extrahepatic MAT typically weighs around 48 kDa. Both enzymes differ in their tissue expression: MAT1A is predominantly found in the liver whereas MAT2A expresses across various tissues such as the brain and intestines.
Biological function summary
MAT2A and MAT1A are key in the methionine cycle impacting gene regulation through methylation reactions. They facilitate the synthesis of SAM a universal methyl donor essential for transmethylation processes within cells. Specifically MAT1A forms a complex with glycine N-methyltransferase to regulate homocysteine and methylation homeostasis. Both enzymes perform a role in controlling the abundance of SAM which influences various cellular processes including nucleic acid protein and lipid metabolism.
Pathways
MAT2A and MAT1A participate actively in methionine and SAM metabolism. They integrate within the one-carbon metabolism pathway which is critical for methylation processes affecting gene expression and epigenetic regulation. MAT2A interacts with proteins like MAT2B which modulates the activity of MAT2A impacting the balance between SAM and S-adenosylhomocysteine. This pathway interaction is important for maintaining methylation balance and cellular proliferation.
MAT2A and MAT1A relate to liver diseases and cancer reflecting their roles in SAM metabolism. Abnormal function or expression of these enzymes affects SAM levels linking to liver cirrhosis and hepatocellular carcinoma. MAT1A mutations can impair methionine metabolism exacerbating liver conditions. Similarly altered MAT2A expression associates with certain cancers where it modulates cellular methylation states and growth. Proteins like SAM might get affected creating an imbalance that contributes to the pathology of liver disease and oncogenesis.