Abcam, ab178013, Human IL-6 ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests / 10 x 96Tests
Human IL-6 ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human interleukin-6 (IL-6) with a sensitivity of 1.6 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA - 450nm readout; works on any plate reader - Different formats for different needs: 10x 96-well plates for bulk orders and 384-well for higher throughput - Broad sample compatibility – including CSF - Cited in over 210 publications
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma, Cerebral Spinal Fluid,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.6 pg/mL,
Range:7.8 - 500 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Human IL-6 ELISA Kit ab178013 is a rapid single-wash 90-min ELISA kit to measure Human IL-6 in cell culture supernatant, serum, plasma, cerebral spinal fluid. This SimpleStep ELISA® sensitivity is 1.6 pg/mL.
How the assay works
Human IL-6 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant Human IL-6 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit. Recombinant Human IL-6Ra was prepared at 50 ng/mL and 500 pg/mL and assayed for cross reactivity. No cross-reactivity was observed. Recombinant Human IL-6Ra was prepared at 50 ng/mL and 500 pg/mL and tested for interference. No interference with was observed.
Human IL-6 ELISA Kit ab178013 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human IL-6 ELISA kit ab178013
Human IL-6 ELISA kit ab178013 has been used to assess inflammation in different physio-pathological contexts such as obesity
, coronary artery disease
, and rheumatoid arthritis
References:
Shaohong Yu et al. 2023, PMID: 38156278,
Yongshun W. et al. 2023, PMID:38054817,
Yaju Tan et al. 2024, PMID: 37997635
Save your precious samples
Our 384-well Human IL-6 ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human/Monkey IL-6 Antibody Pair - BSA and Azide free
ab243973
- Anti-IL-6 antibody [IL-6-10] - BSA and Azide free (Capture)
ab244758
- Anti-IL-6 antibody [IL-6-5] - BSA and Azide free (Detector)
ab244886
Related and recommended products
Human IL-6 ELISA kit ab178013 is commonly used to measure IL-6 as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Human TNF alpha ELISA kit
ab181421
- Human IL-8 ELISA kit
ab214030
- Human IL-1 beta ELISA kit
ab214025
In glioblastoma, IL-6 is known to activate several pro-oncogenic signaling pathways.
Explore our comprehensive range of glioblastoma research solutions here: https://www.abcam.com/en-us/products/product-recommendations/glioblastoma-research-tools
In Alzheimer's disease, increased levels of IL-6 in the plasma or cerebrospinal fluid of patients is associated with cognitive impairment.
Explore our extensive Alzheimer's research solutions here: https://www.abcam.com/en-us/products/product-recommendations/alzheimers-disease-tools

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C