Abcam, ab178653, Anti-Rheumatoid Factor IgM ELISA Kit

Size: 1 x 96Tests
Anti-Rheumatoid Factor IgM ELISA Kit is an indirect ELISA for the qualitative detection of IgM class antibodies against Rheumatoid Factor in human plasma and serum samples. - Colorimetric readout - 450 nm - Works on any standard plate reader - Easy results interpretation
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Assay type:Indirect,
Sensitivity:< 0.5 U/mL,
Assay Platform:Microplate

Product details:
Abcam's anti-Rheumatoid Factor IgM ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of autoantibodies to Rheumatoid Factor IgM in Human serum or plasma.
The quantitative immunoenzymatic determination of IgM rheumatoid factors is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strips are precoated with Fc fragments of Human immunoglobulin G to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added. This conjugate binds to the captured rheumatoid factors. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of IgM rheumatoid factors in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rheumatoid Factor IgM often referred to as IgM RF is an autoantibody that targets the Fc region of IgG immunoglobulins. It is primarily expressed in synovial fluid and blood plasma. The mass of the IgM RF antibody typically ranges between 970-1000 kDa. Its main role involves forming immune complexes by binding to IgG contributing to inflammation and tissue damage in autoimmune conditions.
Biological function summary
The role of Rheumatoid Factor IgM involves mediating immune responses by participating in the formation of antigen-antibody complexes. Although it is not a part of a complex by itself IgM RF can interact with other components of the immune system to exacerbate inflammatory reactions. A characteristic feature is its involvement in the activation of the complement system which plays a part in promoting inflammation and autoimmune processes.
Pathways
Rheumatoid Factor IgM influences processes in the complement cascade and the immune response pathway. It interacts with components such as C-reactive protein and immunoglobulin G both involved in the regulation of inflammatory responses. The activities of IgM RF within these pathways contribute to the enhancement and maintenance of chronic inflammation seen in certain autoimmune conditions.
Rheumatoid Factor IgM is most notably associated with rheumatoid arthritis and Sjögren's syndrome. It often correlates with disease severity and progression in rheumatoid arthritis by forming immune complexes that contribute to chronic joint inflammation and damage. In Sjögren's syndrome IgM RF along with anti-SS-A/Ro antibodies plays a role in the pathogenesis and facilitates glandular dysfunction and dryness. Its presence helps in diagnosis and prognosis of these diseases making it a significant biomarker in autoimmune disease management.