Abcam, ab178780, Triglyceride Assay Kit (Fluorometric, Reducing Samples)

Size: 100Test
Triglyceride Assay Kit (ab178780) is a sensitive, easy assay to measure triglyceride concentration in mammalian samples.
Key facts
Detection method:Fluorescent,
Sample types:Saliva, Heparin Plasma, Tissue, Suspension cells, Serum, Adherent cells,
Assay type:Quantitative,
Sensitivity:< 0.4 µM,
Assay time:1h,
Assay Platform:Microplate reader

Product details:
Triglyceride Assay Kit (ab178780) is a sensitive, easy assay to measure triglyceride concentration in mammalian samples. In this triglyceride assay protocol, triglycerides are hydrolyzed to glycerol and fatty acid. The glycerol reacts with Triglyceride Enzyme Mix to form an intermediate product which in turn reacts with the probe and developer to generate fluorescence (Ex/Em 535/587 nm). The generated fluorescence is directly proportional to the amount of triglycerides.
Triglyceride Assay Kit (Fluorometric, Reducing Samples) ab178780 is suitable for measuring triglyceride levels in samples which contain reducing substances that may interfere with oxidase-based assays such as Triglyceride Assay Kit (
ab65336
Triglyceride assay protocol summary:
- add samples and standards to wells
- add lipase and incubate for 20 min
- add reaction mix and incubate for 30 min
- analyze with a microplate reader.
Please note: the general range is 0-10 nmol (colorimetric) and 0-1 nmol (fluorometric).
How other researchers have used Triglyceride Assay Kit ab178780
The Triglyceride assay kit has been used in publications in a variety of sample types, including:
- Human: Hepatocyte cell/cell line extract
- Mouse: Plasma
, Skeletal muscle extract
, Serum
References: 1-Liu Q et al. 2021, PMID: 34341705, 2-Hu LK et al. 2021, PMID: 34707711, 3-Panajatovic MV et al, PMID: 34066911, 4-Panajatovic MV et al, PMID: 34066911, 5-Maier JI et al. 2021, PMID: 34203913
Other Notes
This product was previously called K614 PicoProbe Triglyceride (TG) Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
Triglycerides are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. Triglycerides are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of triglycerides are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Triglycerides also known as triacylglycerols serve as the major form of fat storage in the body. Composed of three fatty acid chains attached to a glycerol backbone these molecules possess a molecular weight that varies depending on the fatty acid composition. Triglycerides are biosynthesized in liver cells and adipocytes. They accumulate in lipid droplets within these cells providing an energy reserve that organisms can access when necessary.
Biological function summary
Triglyceride molecules play an important role in energy metabolism and storage. Acting within the lipid storage complex these molecules ensure that energy derived from food is efficiently stored and made available for future energy demands. When the body requires energy an enzyme called lipase breaks down triglycerides into free fatty acids and glycerol which can then enter metabolic pathways to be used as cellular fuel.
Pathways
Triglycerides are integral to lipid metabolism and energy production. In lipid metabolism lipase converts triglycerides to free fatty acids and glycerol aiding in their mobilization across cellular membranes. These molecules integrate into the beta-oxidation pathway one of the important metabolic routes for energy production. Proteins such as hormone-sensitive lipase and adipose triglyceride lipase modulate their mobilization coordinating the release and utilization of energy reserves based on physiological needs.
Triglycerides have strong associations with hyperlipidemia and cardiovascular diseases. Elevated triglyceride levels in the body surpassing the normal triglyceride range increase the risk of such conditions. Hyperlipidemia characterized by high triglyceride or cholesterol levels often involves interactions with apolipoproteins which influence lipid transport and deposition. Cardiovascular diseases link to the buildup of lipoproteins of which triglycerides form a substantial part contributing to plaque formation in arterial walls.