Abcam, ab180151, Anti-POLR2E/rpb5 antibody [EPR11750]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal POLR2E/rpb5 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11750,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
POLR2E also known as RPB5 is a subunit of RNA polymerase II which plays a central role in the transcription of DNA into RNA. It weighs approximately 25 kDa. POLR2E is expressed in ubiquitously in human tissues indicating its essential role in basic cellular functions. As a component of the core RNA polymerase II complex it interacts with other polymerase subunits to form the enzyme that synthesizes messenger RNA (mRNA) precursors and many functional non-coding RNAs.
Biological function summary
The function of POLR2E contributes to transcription initiation and elongation processes. It is part of the larger multiprotein complex of RNA polymerase II which consists of twelve different subunits. This complex is responsible for transcribing mRNA from DNA templates in eukaryotic cells. POLR2E enables the assembly of the transcriptional machinery significantly affecting the regulation of gene expression.
Pathways
POLR2E operates in the transcription and mRNA processing pathways. It plays an important role in the synthesis of RNA transcripts that undergo splicing polyadenylation and capping before they become mature mRNAs for protein synthesis. POLR2E interacts with other factors in the transcription complex such as TFIIF and TFIIE which modulate transcriptional efficiency and fidelity.
POLR2E has been linked to transcription-related pathologies such as certain cancers and neurological disorders. Alterations in the expression or function of POLR2E can affect the proper transcription process leading to disrupted cellular functions. It also connects to proteins like BRCA1 in breast cancer where changes in gene expression play a role in tumor development and progression.