Abcam, ab180508, Anti-VAM1 antibody [EPR14157(B)]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal VAM1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14157(B),
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
VAM1 also known as Vacuolar Assembly Machinery Protein 1 is an important component involved in vacuolar membrane fusion and transport processes within cells. The protein has an approximate molecular mass of 55 kDa. It is expressed in various cell types and tissues predominantly within eukaryotic cells where vacuolar functions are critical. In yeast cells VAM1 locates primarily at the vacuolar membrane playing a direct role in vesicle docking and fusion events that are critical for maintaining cellular homeostasis.
Biological function summary
The machinery orchestrated by VAM1 facilitates important vacuolar fusion processes ensuring appropriate cellular trafficking and compartmentalization. VAM1 operates as a part of a larger fusion complex which also includes SNARE proteins and other fusion mediators. Through these interactions VAM1 contributes to the remodeling of vacuolar structures ensuring efficient intracellular transport. Its function affects not just the integrity of vacuolar membranes but also the turnover and degradation of cellular components within the vacuole.
Pathways
VAM1 plays an important role in the homotypic fusion and protein sorting (HOPS) pathway which is important for vacuole docking and fusion. It also influences the endosomal sorting complexes required for transport (ESCRT) pathway where it collaborates with components such as Vps proteins to direct vesicle transport and sorting. These pathways are essential for cellular functions like nutrient recycling and waste degradation processes that significantly impact cell survival and adaptation.
Mutations or dysfunctions in VAM1 can relate to lysosomal storage disorders and neurodegenerative diseases. These conditions can arise due to disruptions in cellular waste processing and protein degradation where VAM1's role in membrane fusion becomes compromised. Furthermore VAM1 interacts with other proteins involved in these processes like the Vps class proteins which also link to various cellular pathologies when their function is impaired. Understanding VAM1 and its network is therefore important for unraveling the molecular basis of these diseases.