Abcam, ab180607, Anti-NPM1/ALK antibody [EPR11413]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal ALK antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11413,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:In more than 50% of systemic Anaplastic large cell lymphoma, t(2;5)(p23;q35) translocation can be found (Falini et al., 1999; Morris et al., 2001). (PMID: 9736036 and PMID: 11380391). The corresponding genes have been cloned and represent a fusion between the NPM and the ALK genes (Morris et al., 1994). (PMID: 8122112). Encodes a chimeric 80-kDa protein consisting of the N-terminal portion (amino acids 1-117) of NPM fused to the cytoplasmic portion of ALK (amino acids 1058-1620). Reference: Galietta et al., 2007. (PMID: 17537995).

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NPM1/ALK fusion protein results from the abnormal fusion of the nucleophosmin (NPM1) and anaplastic lymphoma kinase (ALK) genes. Commonly referred to as NPM-ALK this chimeric protein exhibits a molecular mass of approximately 80 kDa. It expresses mainly in hematopoietic tissues and various tumors. NPM-ALK retains the oligomerization domain of NPM1 and the kinase domain of ALK leading to constitutive activation of the ALK signaling pathway.
Biological function summary
The NPM/ALK fusion protein alters cellular proliferation and survival through oncogenic signaling. The protein does not function alone but forms complexes within cells effectively hijacking normal cellular mechanisms. NPM-ALK cooperates with several signaling molecules including STAT3 and PI3K to promote unchecked cellular growth. This partnership modifies downstream signaling events fostering an environment conducive to malignancy.
Pathways
NPM/ALK activates several key signal transduction pathways. Particularly it plays important roles in the JAK/STAT and PI3K/AKT pathways. Through these pathways the fusion protein engages with STAT3 and AKT catalyzing alterations in gene expression related to cell cycle progression and apoptosis inhibition. These interactions contribute to its oncogenic potential and the pathophysiology of related conditions.
NPM/ALK is intimately associated with anaplastic large cell lymphoma (ALCL) and other hematological malignancies. The inappropriate expression and activity of NPM-ALK disrupts normal cellular functions leading to oncogenesis. This relation extends to its involvement with proteins like STAT3 and others in related pathways enhancing tumor development and progression in ALCL. Therefore understanding NPM-ALK's role provides valuable insight into targeted therapeutic strategies for treating related malignancies.