Abcam, ab181957, Recombinant Dengue Virus 4 NS1 glycoprotein

Size: 100µg
Recombinant Dengue Virus 4 NS1 glycoprotein is a Dengue virus 4 Dominica/814669/1981 Full Length protein, in the 775 to 1126 aa range, expressed in HEK 293 cells, with >95%, suitable for SDS-PAGE, ELISA.
Key facts
Purity:>95% SDS-PAGE,
Expression system:HEK 293 cells,
Tags:His tag C-Terminus,
Applications:ELISA, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P09866,
Animal free:No,
Carrier free:No,
Species:Dengue virus 4 Dominica/814669/1981,
Storage buffer:pH: 7 - 8Constituents: Tris, 0.87% Sodium chloride

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Dengue Virus 4 NS1 glycoprotein also known as non-structural protein 1 plays an important role in the viral replication and immune evasion mechanisms of the Dengue virus. Weighing approximately 46-55 kDa NS1 exists both as a monomer and a dimer displaying versatility in its function. It is expressed in infected host cells and can be secreted into the bloodstream or be present in cellular membranes. This flexibility allows it to interact directly with various elements of the host cell system contributing to its pathogenicity.
Biological function summary
NS1 glycoprotein in Dengue Virus 4 is integral to viral replication and immune modulation. It does not work alone but rather functions as part of a larger complex with other viral proteins to effectively hijack host cellular machinery. NS1 is involved in the formation of replication complexes that along with its immunomodulatory activities suppress host immune responses. Importantly NS1's ability to disrupt the complement system highlights its role in immune evasion and pathogenesis.
Pathways
Several aspects of NS1 function link to host inflammatory and immune response pathways. NS1 interacts within the viral replication pathway engaging with the host's innate immune response. Proteins such as NS2B and NS3 closely relate to NS1 through these pathways functioning together to facilitate efficient virus replication and modulation of host defenses. NS1 contributes to the manipulation of the immune signaling pathways supporting the virus's resistance to host immune clearance mechanisms.
The role of NS1 glycoprotein in Dengue Virus 4 connects it to diseases such as Dengue Fever and Dengue Hemorrhagic Fever. NS1 levels in the bloodstream correlate with disease severity making it a marker for diagnosis and prognosis. Through its interaction with other viral proteins including E protein and NS3 NS1 affects host vascular permeability leading to hemorrhagic manifestations in severe disease cases. The molecular interactions highlight NS1's potential as a target for therapeutic intervention and vaccine development.