Abcam, ab183022, Anti-NELF antibody [EPR8976(2)]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal NELF antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8976(2),
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NELF also known as Negative Elongation Factor consists of four subunits with an approximate molecular mass of 200 kDa. NELF is expressed in various tissues and plays a mechanical role in RNA polymerase II pausing during early transcription elongation. NELF temporarily halts the transcription process by binding the elongation complex affecting the progression of gene expression.
Biological function summary
The interaction of NELF with other initiation factors ensures proper RNA synthesis regulation. NELF forms a part of a complex with DSIF (DRB Sensitivity-Inducing Factor) and RNA polymerase II stabilizing the paused state of transcription. This regulatory action assists in controlling the timing of gene expression and allows other regulatory factors to modify transcription machinery or the chromatin environment.
Pathways
NELF participates in transcription regulation pathways including the c-myc pathway and the heat shock response. In the context of the c-myc pathway NELF works alongside proteins like P-TEFb which helps transition RNA polymerase II from the paused state to productive elongation. This cooperation ensures a responsive and tightly regulated transcription process.
Several cancers and cardiac diseases link to the improper regulation of NELF. Dysregulation of NELF and P-TEFb interactions correlates with aberrant transcription elongation control contributing to tumor development. Similarly disruptions in the heat shock response pathway can lead to cardiac disorders with HSF1 (Heat Shock Factor 1) interacting with NELF during stress response regulation.