Abcam, ab183250, Recombinant Human 4E-BP3 protein

Size: 50µg
Recombinant Human 4E-BP3 protein is a Human Full Length protein, in the 1 to 100 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>85% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:O60516,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Product details:
This product was previously labelled as EIF4EBP3

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
4E-BP3 also known as eukaryotic translation initiation factor 4E-binding protein 3 regulates protein synthesis by binding to eukaryotic initiation factor 4E (eIF4E). It is part of the 4E-BP family which also includes 4E-BP1 and 4E-BP2. The molecular weight of 4E-BP3 is approximately 13 kDa. This protein expresses broadly across various tissues with notable expression in the liver and adipose tissues.
Biological function summary
4E-BP3 regulates the initiation of translation by inhibiting eIF4E which is necessary for cap-dependent translation. It acts as a suppressor for protein synthesis during cellular stress or nutrient deprivation. 4E-BP3 does not form a complex with other proteins but functions as part of the mTOR signaling pathway which governs cell growth proliferation and metabolism.
Pathways
4E-BP3 operates within the mTOR signaling pathway a major regulator of cellular growth and metabolism. It plays a role in the regulation of the PI3K/Akt/mTOR pathway. 4E-BP3 directly interacts with eIF4E blocking the formation of the eIF4F complex and inhibiting the translation of specific mRNAs involved in cell growth and survival. The mTOR pathway integrates signals such as nutrients and energy status impacting overall protein homeostasis.
Dysregulation of 4E-BP3 has been linked to conditions like cancer and obesity. In cancer 4E-BP3's malfunction may lead to uncontrolled protein synthesis contributing to tumor growth and progression. Its relationship with mTOR and eIF4E places it among potential targets for cancer therapeutics. In obesity dysfunctional 4E-BP3 expression can affect fat metabolism and energy balance indicating its role in metabolic disorders.