Abcam, ab183291, TripleStain IHC Kit: M&R&G on human tissue (DAB, AP/Red & DAB/Ni)

Size: 6mL / 18mL / 60mL
Abcam's TripleStain IHC Kit (ab183291) is designed to be used with user-supplied goat, mouse and rabbit primary antibodies to detect three distinct antigens on a single human tissue or cell samples.
Key facts
Assay type:Polymer Multiplex

Product details:
Abcam's TripleStain IHC Kit (ab183291) is designed to be used with user-supplied goat, mouse and rabbit primary antibodies to detect three distinct antigens on a single human tissue or cell samples. This kit has been tested on paraffin embedded tissue; however it may also be used on frozen or freshly prepared monolayer cell smears.
Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183291) supplies polymer enzyme conjugates: Goat HRP Polymer, Rabbit AP Polymer and Mouse HRP Polymer with three chromogens, DAB (brown); Permanent Red (red); and DAB-Ni (black). Abcam's TripleStain IHC Kit (ab183291) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross reaction when detecting three different primary antibodies using our unique blocking system. Simplified steps allow the users to complete triple staining within 3 hours (without antigen retrieval) or 4 hours (with antigen retrieval). The well tested protocol allows the user to permanently mount slides with a coverslip.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C