Abcam, ab183292, TripleStain IHC kit: M&M&R on rodent tissue (DAB, HRP/Green & AP/Red)

Size: 6mL / 18mL / 60mL
The TripleStain IHC kit (ab183292) is designed to use with two mouse antibodies and one rabbit antibody (user supplied) to detect three distinct antigens on a single mouse/rat tissue or cell sample.
Key facts
Assay type:Polymer Multiplex

Product details:
The TripleStain IHC kit (ab183292) is designed to use with two mouse antibodies and one rabbit antibody (user supplied) to detect three distinct antigens on a single mouse/rat tissue or cell sample. This kit has been tested in paraffin-embedded formalin-fixed tissues. However, it can be used on frozen specimens and freshly prepared monolayer cell smears.
Triple staining is a common method used in immunohistochemistry for the detection of three distinct antigens in a single tissue. Abcam's TripleStain IHC Kit supplies polymer enzyme conjugates: polymer-HRP anti-mouse IgG, polymer-AP anti-mouse IgG, and polymer-HRP anti-rabbit IgG with three substrates/chromogens; DAB (brown), Emerald (green), and Permanent Red (Red). DAB (Brown) and Permanent Red (red). The TripleStain Kit is a non-biotin system avoiding non-specific background due to endogenous biotin binding. A Primer step is used to increase specificity of antibody staining. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using a unique blocking system.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C