Abcam, ab183295, TripleStain IHC Kit: R&R&M on rodent tissue (DAB , AP/Red & HRP/Green)

Size: 6mL / 18mL / 60mL
Abcam's TripleStain IHC Kit (ab183295) is designed to be uses with user-supplied one mouse and two rabbit primary antibodies to detect three distinct antigens on a single mouse/rat tissue or cell sample.
Key facts
Assay type:Polymer Multiplex

Product details:
Abcam's TripleStain IHC Kit (ab183295) is designed to be uses with user-supplied one mouse and two rabbit primary antibodies to detect three distinct antigens on a single mouse/rat tissue or cell sample. This kit has been tested on tissue specimens that are paraffin embedded; however it may also be used on frozen or freshly prepared monolayer cell smears.
Triple staining uses traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183295) supplies polymer enzyme conjugates: Mouse HRP Polymer, Rabbit HRP Polymer and Rabbit AP Polymer with three substrates/chromogens; DAB (brown), Emerald (green), and Permanent Red (Red). Abcam's TripleStain IHC Kit (ab183295) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using a unique blocking system. Simplified steps allow the user to complete triple staining within 5 hours (without antigen retrieval) or 6-7 hours (with antigen retrieval). The well tested protocol allows the user to permanently mount slides with coverslip.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C