Abcam, ab183297, TripleStain IHC Kit: M&R&Rt on rodent tissue (DAB, HRP/Green & AP/Red)

Size: 6mL / 18mL / 60mL
The TripleStain IHC kit (ab183297) is designed to be used with user supplied mouse, rat and rabbit primary antibodies to detect three distinct antigens on mouse tissue and cell samples or human tissue.
Key facts
Assay type:Polymer Multiplex

Product details:
The TripleStain IHC kit (ab183297) is designed to be used with user supplied mouse, rat and rabbit primary antibodies to detect three distinct antigens on mouse tissue and cell samples or human tissue. This kit has been tested on paraffin embedded tissue, however it can also be used on frozen tissue or cell smears.
Triple staining is a common method used in immunohistochemistry for the detection of three distinct antigens and their co-expression in a single tissue. Abcam's TripleStain IHC Kit supplies polymer enzyme conjugates: polymer-HRP anti-mouse IgG, polymer-AP anti-rat IgG, and polymer-HRP anti-rabbit IgG with three substrates/chromogens; DAB (brown), Emerald (green), and Permanent Red (Red). The TripleStain Kit is a non-biotin system avoiding non-specific background due to endogenous biotin binding. This kit has been optimized to have no cross detection when detecting two primary antibodies from the mouse and rat host species using a unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6-7 hours (with antigen retrieval). This protocol also includes a method to dehydrate, clear and permanently mount slides with coverslip.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C