Abcam, ab183362, Human KGF ELISA Kit

Size: 1 x 96Tests
Human KGF ELISA Kit is a single-wash 90-min Simplestep used to quantify Human KGF with a sensitivity of 7.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 7.2 pg/mL,
Range:31.25 - 2000 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
Human KGF ELISA Kit ab183362 is a rapid single-wash 90-min Sandwich ELISA to measure Human KGF in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 7.2 pg/mL.
How the assay works
Human KGF SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human KGF ELISA Kit ab183362 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Human Keratinocyte Growth Factor (KGF) is a 28 kDa glycoprotein and the seventh discovered member of the FGF family (FGF-7). It is primarily secreted by cells of mesenchymal origin and it acts in a paracrine fashion on cells expressing the tyrosine kinase receptor FGFR2b. Binding to the receptor occurs in the presence of heparin sulphate glycosaminoglycans leading to the formation of KGF-FGFR2b-HS dimmers which allow trans-phosphorylation of specific cytoplasmic kinases. Signaling through FGFR2b has a dual effect, whereby it phosphorylates proteins that promote growth stimulation (FRS2-alpha, IRS4, IGF-1, ERK2, SHP2), as well as proteins that attenuate cell growth and promote cell differentiation (Lamina-associated polypeptide 2, SAP102 and DIg family of tumor suppressor proteins). Furthermore, KGF also induces anti-apoptotic pathways through AKT and p21-activated protein kinase 4 (PAK4) and up-regulates multiple genes involved in proliferation, migration, angiogenesis, differentiation, survival, nucleotide biosynthesis, tissue remodeling, cell cycle progression, DNA repair and detoxification of reactive oxygen species. All these molecular processes lead to tissue protection and regeneration.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C