Abcam, ab184860, Human PDGF BB ELISA Kit

Size: 1 x 96Tests / 10 x 96Tests
Human PDGF BB ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PDGF BB with a sensitivity of 2.9 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture media, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.9 pg/mL,
Range:11.7 - 750 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
Human PDGF BB ELISA Kit ab184860 is a rapid single-wash 90-min sandwich ELISA kit to measure Human PDGF BB in Plasma (Citrate, EDTA, and Heparin), Cell culture media, and Serum. This Simplestep sensitivity is 2.9 pg/mL.
How the assay works
Human PDGF BB SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
This immunoassay is calibrated against a highly purified human PDGF BB. The NIBSC/WHO unclassified purified human PDGF BB preparation 94/728 was evaluated in this kit.
The dose response curve of the unclassified standard PDGF BB parallels the SimpleStep standard curve.
To convert sample values obtained with the SimpleStep Human PDGF BB kit to approximate NIBSC IU/mL units, use the equation below.
NIBSC (94/728) approximate value (IU/mL) = 0.0114 x SimpleStep Human PDGF BB value (pg/mL).
Does not react with human PDGF-A.
Human PDGF BB ELISA Kit ab184860 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
How other researchers are using Human PDGF BB ELISA Kit ab184860
Human PDGF BB ELISA Kit ab184860 has been used to study factors influencing efficacy of plasma infusion in reproductive medicine
, characterization of growth factors expressed in head and neck squamous cell carcinomas
, and PDGF BB signaling regulation as a therapuetic strategy in ankylosing spondylitis
References:
P. Lin, et. al., 2023, PMID: 37763186
J. Zhou, et. al., 2022, PMID: 36544698
S. Jo, et. al., 2023, PMID: 36422470
Related and Recommended products
Human PDGF BB ELISA Kit ab184860 is commonly used to measure PDGF BB as a growth factor essential in regulation of embryonic development, cell proliferation, cell migration, survival, and chemotaxis.
Other ELISA kits to measure related growth factors include:
- Human PDGF BB ELISA Kit, Fluorescent
ab229418
- Human PDGF B ELISA Kit
ab300321
- Human PDGF AA ELISA Kit
ab284616
- Human PDGF-CC ELISA Kit
ab234568
- Human PDGF R alpha ELISA Kit
ab242246
- Human PDGFRB ELISA Kit
ab252357
Platelet derived growth factor B chain plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. As a homodimer it is a potent mitogen for cells of mesenchymal origin and is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. PDGF-BB is essential for normal blood vessel development, for normal development of kidney glomeruli and in wound healing. Signaling is modulated by the formation of heterodimers with PDGF A chain to yield PDGF-AB.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PDGF-B also known as platelet-derived growth factor subunit B or PDGF-BB is a critical protein involved in various cellular processes. Composed of 241 amino acids PDGF-B exhibits a molecular mass of approximately 27 kDa. Scientists recognize this protein for its expression primarily in platelets but it also appears in a range of other tissues including smooth muscle cells and fibroblasts. The PDGF-B protein plays a substantial role in the regulation of growth and division inducing pathways important for cellular proliferation.
Biological function summary
PDGF-B influences cellular signaling processes and facilitates interactions that are a part of the PDGF receptor system. PDGF-B can form a homo-dimerization commonly known as the PDGF-BB dimer which is an active form. When PDGF-B binds to its receptor specifically the PDGF receptor β (PDGFR-β) it triggers pathways that control various cellular activities including growth and survival. The interaction of PDGF-B with the receptor is an important step that modulates several downstream signaling pathways.
Pathways
PDGF-B activates significant pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways play a major role in the regulation of cell proliferation and differentiation. Through these pathways PDGF-B exhibits interactions with proteins like Ras and Akt which are integral in transmitting signals within the cell. These cascades result in the promotion of processes such as angiogenesis and wound healing highlighting the importance of PDGF-B in cellular function.
Researchers have linked PDGF-B to pathologies such as cancer and atherosclerosis. For instance overexpression of PDGF-B can lead to abnormal vascular growth contributing to tumor development in cancers. It also plays a role in the progression of atherosclerosis by promoting the migration and proliferation of smooth muscle cells leading to plaque formation. In these contexts PDGF-B shows interaction with other proteins such as VEGF in tumor angiogenesis and LDL in atherosclerotic developments reinforcing its involvement in these conditions.