Abcam, ab184862, Human MIP2 ELISA Kit (CXCL2)

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Human MIP2 ELISA Kit (CXCL2) is a single-wash 90-min Simplestep used to quantify Human MIP2 (CXCL2) with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 10 citations
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 1 pg/mL,
Range:2.3 - 150 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
Human MIP2 ELISA Kit (CXCL2) ab184862 is a rapid single-wash 90-min Sandwich ELISA to measure Human MIP2 (CXCL2)in cell culture supernatant, plasma, serum. This SimpleStep sensitivity is 1 pg/mL.
How the assay works
Human MIP2 (CXCL2)SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human MIP2 ELISA Kit (CXCL2) ab184862 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Macrophage inflammatory protein 2 (MIP2) otherwise known as CXCL2 GRO-beta| or Hematopoietic synergistic factor is a 7.9 kDa heparin-binding chemokine that has potent effects in the response to inflammation and induction of peripheral tolerance. MIP2 shares 70% sequence similarity with mouse and rat MIP2. It is secreted by activated monocytes neutrophils and inflamed mucosal epithelial cells in response to inflammatory stimuli such as IL-1β. MIP2 recruits granulocytic neutrophils and macrophages at sights of inflammation| and causes degranulation of these effector cells at the inflammatory site. It has also been hypothesized that MIP2 acts to synergize the effects of Granulocyte macrophage colony-stimulating factor (GM-CSF) and Macrophage colony-stimulating factor (M-CSF) leading to a larger recruitment of neutrophils and macrophages at the site of inflammation. Research has also suggested that MIP2 is involved in tolerogenic pathways as a lack of the MIP2 receptors CXCR1 and CXCR2 are associated with peripheral tolerance. Clinically. MIP2 is being used as a bone marrow transplant regimen to mobilize peripheral blood hematopoietic stem cells (PBSCs). Mobilization by MIP2 therapy in combination with G-CSF has been associated with faster immune cell reconstitution after transplantation.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The CXCL2 protein also known as macrophage inflammatory protein 2 (MIP-2) or KC in the mouse model functions as a chemokine. It weighs approximately 7.8 kDa and is part of the CXC chemokine family. CXCL2 is mainly expressed in macrophages neutrophils and certain epithelial cells. It plays an important role in mediating the migration of these cells to sites of inflammation or injury.
Biological function summary
CXCL2 attracts and activates neutrophils as part of the innate immune response. It acts independently not as part of a larger complex to provoke a chemotactic response guiding neutrophils to areas of tissue injury. CXCL2 also promotes the release of other cytokines and enzymes that contribute to inflammation. Its activity is mediated through its interaction with receptors like CXCR2 on target cell surfaces.
Pathways
CXCL2 is involved in inflammatory signaling and leukocyte migration. It holds importance in the chemokine signaling pathway which regulates leukocyte trafficking. CXCL2 interacts with proteins like CXCR2 influencing the inflammation process by activating downstream kinases and other signal transduction molecules such as mitogen-activated protein kinases (MAPKs).
CXCL2 is connected to inflammatory conditions and diseases like sepsis and inflammatory bowel disease (IBD). Elevated levels of CXCL2 can enhance the inflammatory response which is linked to the progression of these conditions. The protein is also associated with tumor necrosis factor-alpha (TNF-alpha) which amplifies inflammation and tissue damage during disease processes.