Abcam, ab185039, Alexa Fluor® 488 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker

Size: 100µL
Rabbit Recombinant Monoclonal EEA1 antibody - conjugated to Alexa Fluor® 488. Early Endosome marker. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4245,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EEA1 (Early Endosome Antigen 1) is an important protein involved in endosomal trafficking with a molecular weight of approximately 162 kDa. It is a cytoplasmic protein expressed in various cell types acting mainly as an endosome marker. This protein contains a FYVE domain which facilitates binding to phosphatidylinositol 3-phosphate on endosomal membranes. EEA1 also interacts with Rab5 a small GTPase contributing to the fusion of early endosomes.
Biological function summary
EEA1 plays a significant role in early endosomal pathways facilitating endocytic vesicle fusion. EEA1 does not usually work alone; it functions as part of a larger complex involving other endosomal proteins. This protein acts as a tethering molecule which stabilizes the interaction of vesicles with endosomes. By anchoring vesicles EEA1 ensures the correct delivery and sorting of cargo within the cell which is important for cellular homeostasis and signaling.
Pathways
EEA1 contributes to the endocytic pathway and receptor recycling. It ensures proper functioning of the endocytic recycling of receptors such as the epidermal growth factor receptor (EGFR). EEA1-mediated tethering is vital for these pathways and it works closely with proteins like Rab5 which regulate endocytic trafficking. Through these interactions EEA1 affects cellular processes by controlling the flow of signaling receptors and other molecules within the cell.
EEA1 dysfunction is linked to neurodegenerative diseases and cancer. Abnormalities in its expression or function can disrupt endosomal trafficking leading to cellular stress and disease. For example in Alzheimer's disease impaired endosomal function involving EEA1 can contribute to amyloid-beta accumulation. In cancer altered EEA1 activity may contribute to aberrant receptor signaling which can promote unchecked cell division. The interplay with proteins like EGFR demonstrates EEA1's importance in maintaining cellular health and response mechanisms.