Abcam, ab18528, Anti-LAMP2A antibody - Lysosome Marker

Size: 100µg
Anti-LAMP2A antibody - Lysosome Marker (ab18528) is a rabbit polyclonal antibody detecting LAMP2A in Western Blot, IHC-P, ICC/IF . Suitable for Human, Mouse . - Over 170 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Replenishment batches of our polyclonal antibody, ab18528 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab125068.

Product details:
Product Specifications
Anti-LAMP2A antibody - Lysosome Marker (ab18528) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P, WB in human, mouse samples.
Anti-LAMP2A antibody - Lysosome Marker (ab18528) specifically detects LAMP2A (UniProt ID: P13473; Molecular weight: 42kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-LAMP2A antibody - Lysosome Marker (ab18528) has high sensitivity and specificity.
Anti-LAMP2A antibody - Lysosome Marker (ab18528) has been cited over 177 times in peer reviewed journals and is trusted by the scientific community.
Anti-LAMP2A antibody - Lysosome Marker (ab18528) has 16 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LAMP2A also known as lysosome-associated membrane protein 2A functions as a lysosomal marker and plays a role in the lysosome size and structure regulation. Its molecular weight is approximately 45 kDa. This protein is expressed mainly in tissues with high metabolic activity such as liver kidney and heart. LAMP2A is an integral component of the lysosomal membrane and is important for the proper function and maintenance of lysosomes which are cellular organelles involved in the degradation and recycling of macromolecules.
Biological function summary
LAMP2A facilitates chaperone-mediated autophagy (CMA) a specific mechanism for the selective degradation of proteins. It is not part of a larger complex but rather acts independently within the lysosomal membrane. This transport functions through recognizing cytosolic proteins containing a KFERQ-like motif which are then translocated into the lysosome for degradation. LAMP2A upholds cellular homeostasis and is important in adapting to cellular stress by facilitating the turnover of damaged or unnecessary proteins.
Pathways
LAMP2A is intimately involved in the CMA pathway. This pathway is important for regulating protein quality control often linked with other autophagic processes. LAMP2A also interacts with the heat shock cognate protein (HSC70) which assists in unfolding and transporting substrate proteins to lysosomes. Through these interactions LAMP2A helps maintain cellular proteostasis which is essential for cellular function and survival especially during stress conditions.
LAMP2A is connected to lysosomal storage disorders such as Danon disease. This disease is characterized by the accumulation of autophagic vacuoles due to defects in lysosomal function. LAMP2A mutations alter lysosomal degradation capabilities leading to severe cardiomyopathy and myopathy. Additionally LAMP2A has implications in neurodegenerative diseases like Parkinson's disease due to its role in protein degradation pathways. In these contexts malfunction or altered expression of LAMP2A can disrupt cellular clearance processes contributing to pathogenesis along with proteins such as alpha-synuclein.