Abcam, ab185565, Anti-DEPDC5 antibody

Size: 100µL
Rabbit Polyclonal DEPDC5 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DEPDC5 aa 450-550.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human DEPDC5 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.O75140

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DEPDC5 also called DEP domain containing 5 is a protein that plays a significant role in various cellular processes. It belongs to a protein family known for its involvement in signal transduction. DEPDC5 has a mass of about 160 kDa. This protein expresses broadly in many tissues with notable presence in the brain and the liver indicating its potential importance in these organs.
Biological function summary
DEPDC5 acts as a negative regulator of the mTORC1 signaling pathway by being a part of the GATOR1 complex. This complex is involved in nutrient sensing and regulation of cell growth and metabolism. DEPDC5’s role in mTORC1 regulation links it to the control of protein synthesis and autophagy essential processes in maintaining cellular homeostasis. By interacting with other components of the GATOR1 complex DEPDC5 contributes not only to inhibiting mTORC1 activity but also to the broader metabolic balance.
Pathways
DEPDC5 is integral to the mTOR signaling pathway and the amino acid sensing pathways. The protein acts in coordination with other GATOR1 complex components such as NPRL2 and NPRL3 which together monitor nutrient availability and adapt cellular functions accordingly. These pathways highlight DEPDC5’s role in connecting nutrient signals to cellular growth responses showing its influence on critical regulatory networks affecting cell growth and proliferation.
Mutations in DEPDC5 have been linked to certain neurological disorders notably focal epilepsy. The protein’s involvement in the brain points to its connection with proper neural function where its disruption may lead to disease. Researchers have also found related proteins like MTOR and NPRL3 being associated in these epilepsy cases emphasizing the importance of DEPDC5 in neural signaling pathways and its potential as a target for therapeutic approaches.