Abcam, ab185986, Human IL-10 ELISA Kit (Interleukin-10)

Size: 1 x 96Tests / 1 x 384Tests
Human IL-10 ELISA kit (Interleukin-10) is a single-wash, 90-minute SimpleStep ELISA® assay designed for the quantification of human IL-10 with a sensitivity of 1.4 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA - 450 nm readout; works on any plate reader - Different formats for different needs: 384-well for higher throughput - Broad sample compatibility – works with serum, plasma, and cell culture supernatants - Cited in over 30 publications
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.4 pg/mL,
Range:9.4 - 3000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human IL-10 ELISA Kit (Interleukin-10) ab185986 is a rapid single-wash 90-min ELISA kit to measure Human IL-10 in cell culture supernatant, serum, plasma. This SimpleStep ELISA® sensitivity is 1.4 pg/mL.
How the assay works
Human IL-10 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit is designed for the quantification of human IL-10. The standard protein in this kit is mature full length human IL-10. Native signal was detected in cell culture supernatant. Spike protein experiments were used to validate serum and plasma samples. Saliva, milk, CSF, cell extract and tissue extract samples have not bee tested with this kit. 50% pooled serum and plasma samples from healthy donors were measured in duplicate. All values were below the detectable range of this assay. 50% serum from 10 healthy human male donors was measured in duplicate. All values were below the detectable range of this assay.
Human IL-10 ELISA Kit (Interleukin-10) ab185986 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human IL-10 ELISA Kit ab185986
Human IL-10 ELISA Kit ab185986 has been used to assess inflammation in different physio-pathological contexts such as celiac disease
, tuberculosis
, and atherosclerosis
References:
Soroush Sardari et al. 2023, PMID: 38040898,
Hao Zeng et al. 2023, PMID: 37601387,
Youjiang Qiu et al. 2023, PMID: 36820569.
Save your precious samples
Our 384-well Human IL-10 ELISA Kit (Interleukin-10) only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human IL-10 Antibody Pair - BSA and Azide free (Interleukin-10)
ab243976
- Anti-IL-10 antibody [EPR20433-201] - BSA and Azide free (Capture)
ab244835
- Anti-IL-10 antibody [EPR20433-290] - BSA and Azide free (Detector)
ab244889
Related and recommended products
Human IL-10 ELISA Kit ab185986 is commonly used to measure IL-10 as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Human TNF alpha ELISA kit
ab181421
- Human IL-8 ELISA kit
ab214030
- Human IL-6 ELISA kit
ab178013

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C