Abcam, ab186742, Anti-PGK1 + PGK2 antibody [EPR14908]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal PGK1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14908,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phosphoglycerate kinase 1 (PGK1) and phosphoglycerate kinase 2 (PGK2) are enzymes involved in glycolytic processes. PGK1 also known as PGKA has a molecular mass of approximately 45 kDa and is widely expressed in most tissues especially in those requiring high energy. PGK2 alternatively known as testis-specific PGK has a similar molecular mass of about 45 kDa and is mainly expressed in the testis. Both enzymes ensure the important step of catalyzing the transfer of a phosphate group from 13-bisphosphoglycerate to ADP forming 3-phosphoglycerate and ATP.
Biological function summary
Phosphoglycerate kinase 1 and 2 are important for cellular energy production and metabolic homeostasis. PGK1 plays a role in maintaining general cellular energy needs especially in environments with fluctuating oxygen levels. PGK2 as a testis-specific isoform supports spermatogenesis by facilitating glycolysis important for sperm motility. Both enzymes do not form part of a larger enzymatic complex functioning as standalone entities in their respective pathways.
Pathways
Phosphoglycerate kinases participate in the glycolytic pathway which is essential for glucose catabolism and ATP generation. Within glycolysis PGK1 and PGK2 work with other glycolytic enzymes such as hexokinase and pyruvate kinase. Both enzymes help balance cellular redox states through their action in redox and energy production pathways intimately linked to other metabolic states such as those found in oxidative phosphorylation.
Overactivity of PGK1 has associations with cancer where altered glycolytic activity supports rapid tumor growth. Its partner proteins like HIF-1α help mediate these changes in cancer metabolism. Additionally PGK2 deficiency can result in male infertility due to its indispensable role in sperm energy metabolism. There is no prominent disease linked partner for PGK2 outside its glycolytic function within testis tissue.