Abcam, ab187143, Anti-GTF2E2 antibody [EPR14929]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal GTF2E2 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14929,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IP, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target GTF2E2 also known as General Transcription Factor IIE Subunit 2 participates in the initiation of transcription by RNA polymerase II. It plays an important role in the formation of the transcription initiation complex. The protein has a molecular mass of approximately 32 kDa. GTF2E2 is expressed in various tissues with significant expression detected in the human heart and skeletal muscle.
Biological function summary
GTF2E2 interacts with other general transcription factors to promote the assembly of the pre-initiation complex. It forms a part of the TFIIE complex with another subunit TFIIE α. This complex is significant for transcription initiation as it helps in opening the DNA double helix. Its activity influences gene expression regulation and is important for directing RNA polymerase II to specific promoter sites.
Pathways
The protein is involved in the RNA polymerase II transcription initiation pathway a critical step for mRNA synthesis. Additionally it affects the regulation of the p53 signaling pathway often interacting with proteins like TFIIH and TBP. The association with these pathways highlights its role in maintaining the gene expression safety and responding to cellular stress signals.
Altered expression of GTF2E2 correlates with cancer and developmental disorders. Its dysfunction might lead to malformed transcription initiation influencing oncogenic pathways. Mutations or misregulation can affect the stability of the transcription machinery potentially linking it to the BRCA1 protein in breast cancer further highlighting the need for precise transcription control.