Abcam, ab187466, Recombinant Human HEMK2/N6AMT1 protein

Size: 20µg
Recombinant Human HEMK2/N6AMT1 protein is a Human Full Length protein, in the 1 to 214 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>85% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9Y5N5,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.4Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Product details:
This product was previously labelled as HEMK2.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein HEMK2 also known as N6AMT1 is an important enzyme in methylation processes. It possesses a molecular mass of approximately 53 kDa. HEMK2/N6AMT1 functions as a methyltransferase catalyzing the addition of a methyl group to certain substrates. It prominently localizes in the cytoplasm and nucleus of human cells with varying expression across different tissues but generally found in higher levels in the testis and heart. Its role in methylation makes it essential for the regulation of protein synthesis and stability.
Biological function summary
HEMK2/N6AMT1 involves the methylation of specific proteins contributing to protein modifications and cell function regulation. This protein often forms part of a larger complex that facilitates its role in transferring methyl groups onto target proteins which in turn affects their function and interactions. HEMK2/N6AMT1's activity influences processes like translation fidelity by targeting elongation factors for methylation helping maintain cellular homeostasis.
Pathways
HEMK2/N6AMT1 plays a role in epigenetic regulation and protein modifications. It participates in the methylation pathway integrating with processes that impact gene expression and protein interactions. In these pathways HEMK2/N6AMT1 interacts with related proteins such as TRMT112 which acts as an activator and stabilizer. These pathways are important for the control of gene expression showcasing the broad influence HEMK2/N6AMT1 has on cellular functioning by regulating the methylation status of proteins.
The dysregulation of HEMK2/N6AMT1 associates with cancer and intellectual disabilities. Abnormal expression or malfunction can lead to improper gene silencing or activation contributing to oncogenesis or neurological impairments. The protein interacts with other proteins like METTL5 in these contexts highlighting its significance in maintaining methylation balances and cellular health. Understanding HEMK2/N6AMT1's connections within these diseases aids in developing targeted therapies and diagnostic approaches.