Abcam, ab187881, Anti-PRDM14 antibody

Size: 100µL
Rabbit Polyclonal PRDM14 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 18 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PR domain zinc finger protein 14.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:IP, ChIP, ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PR domain zinc finger protein 14. The exact immunogen used to generate this antibody is proprietary information.Q9GZV8

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.3Preservative: 0.09% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRDM14 also known as PR domain zinc finger protein 14 acts as a transcription factor with a mass of approximately 64 kDa. It contains an N-terminal PR (PRDI-BF1 and RIZ) domain and zinc-finger motifs within its structure which participate in DNA binding. The protein expresses highly in embryonic stem cells and primordial germ cells showing a strong presence in pluripotent cell states.
Biological function summary
PRDM14 plays a vital role in the maintenance of pluripotency and regulation of gene expression during early embryonic development. It forms part of a regulatory network that includes other factors like OCT4 and SOX2 promoting stem cell self-renewal and inhibiting differentiation. This protein directly influences the expression of genes related to germ cell formation.
Pathways
PRDM14 interacts with several key signaling pathways notably the stem cell pluripotency pathway and the Wnt signaling pathway. It collaborates closely with proteins such as NANOG and TET proteins modulating gene expression patterns essential for pluripotent stem cell identity. Through these pathways PRDM14 ensures proper cell fate decisions during development.
PRDM14’s dysfunctions associate with certain cancers and reproductive health issues. Aberrant expression or mutation of PRDM14 appear in germ cell tumors as its regulatory functions become disrupted. Additionally PRDM14 may link to infertility disorders due to its role in germ cell development. Alterations in its activity can disrupt interactions with proteins like PRDM1 impacting normal cell proliferation and differentiation.