Abcam, ab188020, Anti-RFX4 antibody

Size: 100µL
Rabbit Polyclonal RFX4 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RFX4 aa 500-650.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human RFX4 aa 500-650. The exact immunogen used to generate this antibody is proprietary information.Q33E94

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RFX4 also known as Regulatory Factor X4 is a transcription factor that plays an important role in gene regulation. The protein has a mass of approximately 74 kDa and is expressed in the brain particularly within the central nervous system. It binds to X-box motifs influencing the transcription of various genes by forming complexes with other regulatory proteins. RFX4 is a member of the RFX transcription factor family which is characterized by a highly conserved DNA-binding domain.
Biological function summary
In the context of genetic regulation RFX4 modulates gene expression playing a role in CNS development. It is part of a transcription complex that works together with CREB binding protein and other transcriptional regulators to influence gene expression patterns. Its activity impacts cellular function by controlling genes essential for neural differentiation and development. Studies show that RFX4 is involved in the development of certain brain structures during embryonic growth.
Pathways
RFX4 contributes significantly to developmental pathways such as the Sonic Hedgehog (SHH) signaling pathway. It interacts with other proteins including GLI transcription factors to contribute to the regulation of target gene expression. RFX4 also participates in the Notch signaling pathway where it associates with other components to influence cell fate decisions during neural development. These interactions establish its role in orchestrating precise molecular processes critical for cellular differentiation and development.
Alterations in RFX4 have been linked to neurological conditions such as hydrocephalus and holoprosencephaly. These disorders often stem from defects in brain development where proper functioning of RFX4 and its interaction with proteins like SHH and CREB binding protein are impaired. Research suggests that abnormal RFX4 activity can disrupt standard pathways contributing to the pathogenesis of these diseases by influencing neural cell development and differentiation.