Abcam, ab188617, Anti-NOLA1 antibody

Size: 100µL
Rabbit Polyclonal NOLA1 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GAR1 aa 50-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human GAR1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.Q9NY12

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NOLA1 also known as NOP58 or nucleolar protein 58 is a core component of the box C/D small nucleolar ribonucleoprotein (snoRNP) complex important in pre-rRNA processing. The NOLA1 protein has a molecular mass of approximately 58 kDa. You will find it expressed mainly within the nucleolus of the cell where it participates in the ribosome biogenesis. The structural integrity of NOLA1 is necessary for its function allowing for interactions with other snoRNP components like fibrillarin and NOP56.
Biological function summary
NOLA1 participates in ribosomal RNA (rRNA) maturation and assembly making it indispensable for the synthesis of ribosomes. As a part of the box C/D snoRNP complex it orchestrates the methylation of rRNA by guiding and stabilizing methyltransferase activity provided by fibrillarin. This activity is essential for the modification of rRNA which is a requisite for proper ribosome assembly and function. The action of NOLA1 directly affects cellular protein synthesis by ensuring ribosomes are correctly assembled.
Pathways
The interactions and functions of NOLA1 mostly impact the ribosome biogenesis pathway. This pathway involves the transformation of pre-rRNA into mature rRNA a process critical for functional ribosome production. As a component of this pathway NOLA1 interacts with proteins like NOP56 and fibrillarin which jointly facilitate the methylation and processing of pre-rRNA. Involvement in these pathways underlines how NOLA1 supports cellular growth and protein synthesis efficiency.
Deregulation or mutations in NOLA1 have implicatory links to specific nucleolar stress conditions and various cancers. Disruptions in its function can lead to improper ribosome biogenesis affecting cancerous growth due to unregulated protein synthesis. NOLA1's role connects to proteins like p53 which monitors nucleolar stress and can trigger apoptotic pathways when stress is detected. This connection highlights its importance in maintaining cellular homeostasis and preventing pathological conditions.