Abcam, ab189520, Anti-XLF antibody [EPR15882-42]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal XLF antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15882-42,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The XLF protein also known as XRCC4-like factor plays an important role in DNA repair mechanisms. It weighs approximately 33 kDa and is expressed in various tissues with high levels noted in the thymus and testis. XLF functions as a part of the non-homologous end joining (NHEJ) pathway where it aids in the repair of DNA double-strand breaks. It acts by promoting the ligation of broken DNA ends often working closely with other proteins such as XRCC4 and DNA ligase IV.
Biological function summary
XLF participates in the critical process of maintaining genomic stability. It is part of the NHEJ complex alongside XRCC4 and DNA ligase IV ensuring efficient repair of DNA breaks. This repair process is essential for preventing genomic instability and protecting cells from deleterious mutations. XLF helps tether DNA ends together providing structural support necessary for effective repair and its function is vital for cell survival especially in rapidly dividing cells.
Pathways
This protein integrates into the NHEJ pathway a primary mechanism for repairing DNA double-strand breaks. XLF collaborates directly with other proteins like Ku70/80 and DNA-PKcs facilitating their functions in the DNA damage response. Additionally it plays a role in the V(D)J recombination process which is vital for the development of immune system diversity by allowing the rearrangement of variable (V) diversity (D) and joining (J) gene segments.
XLF relates to severe combined immunodeficiency (SCID) and neurodegenerative disorders. XLF deficiencies can manifest as immunodeficiency due to impaired V(D)J recombination adversely affecting the immune response. It is also linked to neurodegeneration because proper DNA repair is important for neuronal survival. Other proteins in these contexts include DNA ligase IV which shows connection in supporting similar cellular functions and diseases.