Abcam, ab19035, Anti-Claudin 14 antibody

Size: 100µg
Goat Polyclonal Claudin 14 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CLDN14 aa 200 to C-terminus.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human CLDN14 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O95500

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Claudin-14 (CLDN14) plays an important role in the mechanics of epithelial tight junctions contributing to paracellular transport regulation. It forms a barrier that controls the flow of ions and small molecules between cells. CLDN14 has a molecular weight of around 23 kDa. It expresses mainly in the epithelial cells of various tissues including the kidney where it regulates calcium reabsorption and balance in the body.
Biological function summary
Claudin-14 functions as an integral component of tight junction complexes. It interacts with other claudins occludins and junctional adhesion molecules to maintain the paracellular permeability. This protein contributes to the formation and function of the tight junctional structures ultimately influencing ion selectivity and permeability. Studies have shown its role in maintaining electrolyte composition and osmotic balance in cellular environments.
Pathways
Claudin-14 integrates in the calcium-sensing pathway and the tight junction signaling pathway. It associates with calcium-sensing receptors (CaSR) in the kidneys impacting calcium homeostasis. Tight junction signaling involves interactions with proteins such as ZO-1 which is important for maintaining tight junction integrity and regulating epithelial barriers. These connections ensure the coordination of ion transport and stringent control of cellular environments.
Mutations or dysregulations in Claudin-14 link to familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC) and sensorineural deafness. FHHNC results from impaired calcium reabsorption affecting kidney function and calcium balance. Sensorineural deafness arises due to disorder in ion homeostasis in the inner ear where Claudin-14 is also present. Its interactions with CaSR and other tight junction proteins highlight the importance of Claudin-14 in maintaining specific physiological balances related to these conditions.