Abcam, ab190535, Mouse Retinol Binding Protein ELISA Kit (RBP)

Size: 1 x 96Tests
Mouse Retinol Binding Protein ELISA Kit (RBP) is a Sandwich (quantitative) ELISA for the measurement of Mouse Retinol Binding Protein (RBP) in Mouse in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.152 ng/mL,
Range:0.625 - 20 ng/mL,
Assay Platform:Microplate

Product details:
Abcam's Retinol Binding Protein (RBP) Mouse ELISA kit (ab190535) is an
in vitro
enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Retinol Binding Protein (RBP) in mouse serum and plasma.
In this assay the Retinol Binding Protein (RBP) present in samples reacts with the anti-Retinol Binding Protein (RBP) antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-RBP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound RBP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of RBP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of RBP in the test sample. The quantity of RBP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Retinol Binding Protein (RBP) also known as RBP4 is a small transport protein with a molecular weight of approximately 21 kDa. It plays an essential role in the transport of vitamin A (retinol) throughout the body. RBP expresses mainly in the liver and is released into the bloodstream to carry retinol to various organs. This protein binds with retinol to form a complex that enables safe transportation as retinol is hydrophobic and insoluble in water.
Biological function summary
RBP interacts with the cell membrane receptors to deliver retinol to target cells where it's needed for various physiological processes. It functions either independently or as part of a complex with transthyretin (TTR) in the extracellular space. This interaction with TTR stabilizes RBP and its retinol cargo in circulation and prevents renal clearance. The presence of this complex is significant for maintaining vitamin A homeostasis in the body.
Pathways
RBP serves an important role in retinoid metabolism and transport pathways. In the retinoid pathway RBP ensures the bioavailability of retinol which is further metabolized into active metabolites like retinal and retinoic acid. These metabolites are important for vision and gene regulation. RBP works closely with proteins such as STRA6 (Stimulated by Retinoic Acid Gene 6) a membrane receptor that facilitates retinol uptake into cells further connecting RBP to cellular retinoic acid signaling pathways.
RBP4 has associations with conditions like type 2 diabetes and obesity. Elevated levels of RBP4 in the bloodstream have been linked to insulin resistance a common feature of type 2 diabetes. Studies suggest that these high levels of RBP4 might interfere with insulin signaling contributing to the disease pathogenesis. Another protein of interest in these disorders is adiponectin which inversely correlates with RBP4 levels; as RBP4 increases adiponectin tends to decrease highlighting a potential mechanism by which RBP4 may impact metabolic diseases.