Abcam, ab190545, Rat L-FABP ELISA Kit

Size: 1 x 96Tests
Rat L-FABP ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat L-FABP in Rat in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.278 ng/mL,
Range:3.125 - 200 ng/mL,
Assay Platform:Microplate

Product details:
Abcam's L-FABP Rat ELISA Kit (ab190545) is an
in vitro
enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of L-FABP in rat serum and plasma.
In this assay the L-FABP present in samples reacts with the anti-L-FABP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-L-FABP antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound L-FABP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of L-FABP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of L-FABP in the test sample. The quantity of L-FABP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Liver fatty acid binding protein (liver FABP) also known as L-FABP or FABP1 is a small protein with a mass of approximately 14 kilodaltons. It functions mainly in the liver where it binds free fatty acids and other lipophilic substances facilitating their transport within cells. This protein is highly expressed in hepatocytes and also found in the small intestine and kidneys. Its role in binding fatty acids positions it as an important mediator in lipid metabolism making it of interest in a variety of metabolic studies.
Biological function summary
Liver FABP is essential for maintaining lipid homeostasis within cells. It is not part of a larger complex but acts by itself to regulate the intracellular concentration of lipids and protect cells from lipotoxicity. By sequestering fatty acids it prevents these molecules from disrupting cellular membranes or signaling pathways. Liver FABP also participates in the uptake transport and metabolic conversion of fatty acids and their metabolites influencing energy homeostasis and other vital processes.
Pathways
Liver FABP plays a significant role in the fatty acid metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathways. It acts by modulating the availability of lipid ligands necessary for PPAR activation linking it functionally to these nuclear receptors that control gene expression involved in lipid and glucose metabolism. Liver FABP's association with proteins like FABP2 and FABP4 within these pathways provides insight into its broader metabolic network highlighting its interactions in fatty acid transport and metabolism.
Liver FABP shows a strong connection to metabolic conditions particularly non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes. Its dysregulation is often observed in these disorders which include altered lipid profiles and insulin resistance. Liver FABP is also linked to certain forms of cancer with aberrant expression levels found in some tumor types. The interactions of liver FABP with proteins such as FABP5 in these diseases suggest a potential role in the pathogenesis of metabolic disorders and tumor development making it a candidate for further research and therapeutic targeting.