Abcam, ab190805, Human CXCL1 ELISA Kit (GRO alpha)

Size: 1 x 96Tests / 10 x 96Tests
Human CXCL1 ELISA Kit (GRO alpha) is a single-wash 90-min Simplestep used to quantify Human CXCL1 (GRO alpha) with a sensitivity of 0.064 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders - Validated on a number of sample types including cerebrospinal fluid (CSF) - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.064 pg/mL,
Range:2.34 - 150 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
Human CXCL1 ELISA Kit (GRO alpha) ab190805 is a rapid single-wash 90-min Sandwich ELISA to measure Human CXCL1 (GRO alpha)in cell culture supernatant, CSF, citrate plasma, heparin plasma, serum. This SimpleStep sensitivity is 0.064 pg/mL.
How the assay works
Human CXCL1 (GRO alpha)SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human CXCL1 ELISA Kit (GRO alpha) ab190805 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
CXCL1 (GRO alpha) is a member of the CXC family of chemokines. CXCL1 contains the ELR motif and preferentially chemoattracts neutrophils. Human CXCL1 undergoes post-translational modification into three isoforms called CXCL1(4-73), CXCL1(5-73) and CXCL1(6-73). These isoforms are produced by proteolytic cleavage after secretion from peripheral blood monocytes, and all show higher activity in-vitro than the full-length protein. Two additional CXC proteins, CXCL2 (or GRO beta) and CXCL3 (or GRO gamma), share 90% and 86% amino acid sequence homology, respectively, with CXCL1. Human CXCL1 mRNA is expressed in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, CXCL1 mRNA has been detected in mammary fibroblasts and epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. CXCL1 is a growth-regulated gene and is overexpressed constitutively in tumorigenic cells. As a result, elevated levels of CXCL1 can be seen in several types of tumors and cancers, including lung cancer and melanoma.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CXCL1 also known as GRO alpha or KC in certain species is a chemokine with a molecular mass of around 7.8 kDa. This protein is primarily secreted by macrophages neutrophils and endothelial cells. It plays a critical role in the recruitment of neutrophils during inflammation and acts by binding to the CXCR2 receptor. CXCL1 is expressed in many tissues including lungs liver and skin where it regulates the immune response and facilitates tissue repair.
Biological function summary
CXCL1 functions as a significant mediator in inflammatory processes. It does not form a complex but acts independently to exert its effects. By attracting neutrophils to sites of injury or infection CXCL1 supports the body's defense mechanisms. This chemokine also influences angiogenesis contributing to the formation of new blood vessels which is essential in wound healing and tissue regeneration.
Pathways
CXCL1 plays a role in both the NF-kB signaling and MAPK signaling pathways. These pathways are pivotal for the regulation of immune and inflammatory responses. CXCL1 acts alongside proteins such as IL-8 another chemokine that also binds to the CXCR2 receptor. Through these pathways CXCL1 influences the activation and migration of immune cells facilitating a rapid response to inflammatory stimuli.
CXCL1 has a significant association with conditions such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In COPD elevated levels of CXCL1 contribute to persistent inflammation and neutrophil infiltration. In rheumatoid arthritis CXCL1 participates in joint inflammation and damage interacting with proteins like TNF-alpha which amplifies inflammatory responses. Understanding the role of CXCL1 in these conditions could help guide the development of targeted therapies to mitigate inflammation and tissue damage.