Abcam, ab190814, Human CD31 ELISA Kit (PECAM)

Size: 1 x 96Tests
Human CD31 ELISA Kit (PECAM) is a single-wash 90-min Simplestep used to quantify Human CD31 (PECAM) with a sensitivity of 44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Urine, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human, Rhesus monkey,
Assay type:Sandwich (quantitative),
Sensitivity:= 44 pg/mL,
Range:226.6 - 14500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human CD31 ELISA Kit (PECAM) (ab190814) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD31 (PECAM) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, saliva, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD31 (PECAM) with 44 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CD31, also known as PECAM-1, is a cell adhesion molecule which is required for leukocyte trans-endothelial migration (TEM) under most inflammatory conditions. CD31 is a 130-kDa glycoprotein constitutively expressed on endothelial cells, platelets, neutrophils, monocytes, lymphocytes and basophils. CD31 molecules is expressed in large amounts in the intracellular junctions of endothelial cells. Experiments using antibody results in blocking CD31 homophilic interaction and inhibition of leukocytes transmigration. Expression of CD31 on the cell surface is regulated by receptor shedding resulting in generation of a soluble 100-kDa extracellular domain form and a truncated 28-kDa fragment containing the transmembrane and cytoplasmic domain of CD31.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C