Abcam, ab190982, Anti-TRAM1/TRAM antibody - C-terminal

Size: 100µg
Rabbit Polyclonal TRAM1/TRAM antibody. C-terminal. Suitable for IHC-P, ICC, WB, IHC-Fr and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TRAM1 aa 300-350.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:WB, IHC-P, IHC-Fr, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human TRAM1 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.Q15629

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRAM1 also known as TRAM is a translocation-associated membrane protein involved in the translation and insertion of nascent polypeptides into the endoplasmic reticulum (ER). This protein plays an important role in directing proteins into the ER membrane or lumen via the translocon complex. TRAM1 has a molecular mass of approximately 37 kDa and is widely expressed especially in tissues with high secretory activity such as the liver pancreas and intestine.
Biological function summary
TRAM1 interacts with ribosomes and assists in the co-translational translocation of polypeptides across the endoplasmic reticulum membrane. It forms part of the larger translocon complex which includes Sec61 and other associated proteins. These interactions are necessary for accurate protein targeting and membrane integration ensuring newly synthesized proteins reach their intended cellular compartments.
Pathways
TRAM1 is important for protein secretion and membrane protein biosynthesis pathways. It collaborates closely with Sec61 facilitating protein entry into the ER. It also interfaces with the signal recognition particle (SRP) pathway guiding ribosome-bound nascent chains to the ER membrane. These pathways are essential for proper protein folding processing and eventual function impacting cell signaling and homeostasis.
Mutations or dysfunctions in TRAM1 have potential links to disorders such as congenital disorders of glycosylation and certain liver diseases. Altered interactions with proteins like Sec61 can disrupt normal protein processing pathways contributing to cellular stress and pathology. TRAM1's role in the protein translocation process makes it an important subject of study for understanding the molecular basis of these conditions.