Abcam, ab190994, Recombinant human IRE1b protein

Size: 5µg / 10µg
Recombinant human IRE1b protein is a Human Fragment protein, in the 499 to 926 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab190994 ws determined to be 2.6 nmol/ min/ mg.,
Accession:Q76MJ5,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Product details:
ab64311
(Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The IRE1β also known as ERN1B is a transmembrane endoribonuclease with a mass of approximately 110 kDa. It belongs to the IRE1 family and acts as an important sensor of the unfolded protein response (UPR) pathway. IRE1β is expressed mainly in epithelial cells of the intestine with some presence in lungs and liver tissues. The protein comprises a luminal domain that detects misfolded proteins along with a kinase and RNase domain that initiates downstream signaling pathways.
Biological function summary
IRE1β plays a significant role in cellular stress responses by regulating mRNA splicing and degradation especially during endoplasmic reticulum (ER) stress events. It operates as a part of a complex signal transduction mechanism that maintains cellular homeostasis under stress. In particular upon activation IRE1β engages in unconventional splicing of the transcription factor XBP1 mRNA which in turn upregulates genes involved in protein folding and ER-associated degradation.
Pathways
IRE1β functions as a central component in the UPR pathway influencing ER homeostasis and adaptive stress responses. It works closely with proteins such as PERK and ATF6 which are other transducers of the UPR pathway. This integrated response helps cells to recover from stress by improving protein folding degradation and overall ER function. Additionally IRE1β and its related pathways link to the inflammatory response pathways linking protein misfolding with inflammation signaling.
IRE1β is connected to conditions like inflammatory bowel disease (IBD) and cystic fibrosis. IRE1β modulates intestinal epithelial response to inflammation affecting the pathogenesis of IBD. It also influences the aggregation of misfolded proteins that characterize cystic fibrosis directly associating with proteins like CFTR whose malfunction leads to disease symptoms. Proper regulation of IRE1β activity is vital for maintaining intestinal health and preventing chronic inflammation.