Abcam, ab191000, Recombinant human MYO3A protein

Size: 5µg / 10µg
Recombinant human MYO3A protein is a Human Fragment protein, in the 1 to 434 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>85% Densitometry,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The Specific activity of ab191000 was determined to be 4 nmol/min/mg.,
Accession:Q8NEV4,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Product details:
ab64311
(Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MYO3A also known as Myosin IIIA is motor protein with an approximate mass of 187 kDa. This protein belongs to the class of actin-based motor proteins characterized by ATPase activity facilitating the movement along actin filaments. MYO3A is expressed mainly in the sensory cells of the inner ear and retina playing a role in cellular processes that require specialized cellular projections like stereocilia and photoreceptor cells. Its ability to regulate actin filament dynamics is essential in these sensory tissues.
Biological function summary
MYO3A influences cellular activities through its role in cargo transport and maintaining the organization of actin structures. It operates as part of a protein complex that includes the actin-related protein Arp2/3 which collaborates to promote actin polymerization and branching. Additionally MYO3A interacts with other proteins such as espin-1 accentuating its function in the maintenance and elongation of actin protrusions. This interaction underlines its contribution to the structural integrity necessary for proper sensory cell function.
Pathways
MYO3A is significant in sensory transduction and cell morphogenesis pathways. Its interaction with actin and the Arp2/3 complex places MYO3A within the pathways involved in the regulation of actin cytoskeleton remodeling. MYO3A works closely with other proteins like harmonin critical for organizing the mechanotransductive hair bundle in the inner ear. These connections emphasize the involvement of MYO3A in pathways affecting the maintenance of cellular architecture and signal transmission.
MYO3A mutations are linked to hereditary deafness and Usher syndrome type 3 which involves auditory and visual impairments. In the context of these diseases MYO3A interacts with proteins like cadherin 23 important for hair cell function in the cochlea and usherin associated with retinal cells. These associations highlight MYO3A's importance in sensory cell function and its role in maintaining cellular structures that when disrupted can lead to sensory deficits.