Abcam, ab191208, Anti-Cytokeratin antibody [34BE12]

Size: 500µL
Mouse Monoclonal Cytokeratin 1 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 9 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:34bE12,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:ab191208 recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. ab191208 shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate.

Properties and Storage Information:
Form-Liquid, Purification notes-purified from Bioreactor Concentrates by Protein A/G column chromatography, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytokeratins also known by the abbreviation 'CK' are a class of intermediate filament proteins providing structural support to epithelial cells. Their mass ranges between 40 kDa to 68 kDa. Cytokeratins are expressed in epithelial tissues including the outer layer of skin and line organs such as the gut and respiratory tract. Commonly referenced with specific subtypes CK MNF116 or CK cocktail antibodies are used for detection in laboratory settings. These proteins help maintain cell integrity and protect from mechanical stress.
Biological function summary
Keratins function as a complex to create a stable cytoskeletal network inside epithelial cells. They interact with proteins to form keratin filaments contributing to the mechanical strength and resilience of cells. Cytokeratins also play a role in intracellular signaling and cellular differentiation. They participate in wound healing processes through modulation of cell proliferation and apoptosis. The CK MNF116 marker is especially noted for its application in identifying and characterizing epithelial proteins in pathology.
Pathways
Cytokeratins are involved in pathways related to cell structure and stress response. Specifically they play key roles in the Epidermal Growth Factor (EGF) signaling pathway and the cell cycle control pathway. These pathways involve proteins like epidermal growth factor receptors (EGFR) and cyclins which regulate cell cycle progression. Cytokeratins influence these pathways by impacting cell adhesion and proliferation essential for maintaining epithelial cell functions.
Cytokeratins have significant roles in cancer diagnostics particularly in epithelial tumors such as carcinomas. Altered expression of cytokeratins can indicate the presence and progression of tumors. Cytokeratin profiles including CK MNF116 positive markers provide insights into tumor type and origin. Additionally they relate to liver disorders like cirrhosis where abnormal keratin organization is observed. These proteins show associations with other structural proteins including vimentin in disease states where cell integrity and signaling are often compromised.