Abcam, ab191496, Anti-Mkl2 antibody

Size: 100µg
Rabbit Polyclonal Mkl2 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MRTFB.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MRTFB. The exact immunogen used to generate this antibody is proprietary information.Q9ULH7

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purity >95%., Storage buffer-Preservative: 0.1% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Mkl2 protein also known as Myocardin-like 2 or MRTFB is a transcriptional coactivator that primarily interacts with serum response factor (SRF) to regulate gene expression. It has a molecular weight of approximately 118 kDa. Mkl2 is ubiquitously expressed but shows high levels of expression in heart and skeletal muscles. It predominantly resides in the cytoplasm but translocates to the nucleus upon activation.
Biological function summary
Mkl2 influences cytoskeletal dynamics and cellular motility by modulating actin cytoskeleton organization. It forms a complex with SRF to control the transcription of genes involved in these processes. This complex is essential for responding to environmental cues that promote cell differentiation and proliferation.
Pathways
Mkl2 is integral to the RhoA-MRTFA-SRF signaling pathway which links changes in the actin cytoskeleton to transcriptional responses. Mkl2 works in conjunction with proteins like MKL1 to mediate these pathway interactions. Together they coordinate responses to external stimuli that affect cellular architecture and movement.
Mkl2 has implications in cardiac hypertrophy and cancer progression. Aberrant expression or regulation of Mkl2 can lead to pathological cardiac growth impacting heart function. It also associates with oncogenes in the context of cancer promoting tumor cell invasion and metastasis. Understanding its interactions with proteins like MRTFA in these conditions provides potential therapeutic targets for treatment.